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We have used a chromatin immunopurification approach to identify target genes regulated by the homeotic gene Ultrabithorax. A monoclonal antibody against the Ultrabithorax gene product is used to immunopurify in vivo Ultrabithorax protein binding sites in embryonic chromatin. The procedure gives an enrichment of sequences with matches to a consensus homeodomain binding site. In one case we have shown that an immunopurified sequence lies within a 4 kb fragment that acts in vivo as a homeotic response element. We anticipate that this approach will enable us to identify further targets, allowing the analysis of their regulation and function. The chromatin immunopurification strategy may be of general application for the identification of direct in vivo targets of DNA-binding proteins.
R A White, and
J J Brookman, and
A P Gould, and
L A Meadows, and
L S Shashidhara, and
D I Strutt, and
T A Weaver
August 1994,
Biochemical Society transactions,
R A White, and
J J Brookman, and
A P Gould, and
L A Meadows, and
L S Shashidhara, and
D I Strutt, and
T A Weaver
August 1994,
BioEssays : news and reviews in molecular, cellular and developmental biology,
R A White, and
J J Brookman, and
A P Gould, and
L A Meadows, and
L S Shashidhara, and
D I Strutt, and
T A Weaver
February 1992,
The New biologist,
R A White, and
J J Brookman, and
A P Gould, and
L A Meadows, and
L S Shashidhara, and
D I Strutt, and
T A Weaver
April 1994,
Mechanisms of development,
R A White, and
J J Brookman, and
A P Gould, and
L A Meadows, and
L S Shashidhara, and
D I Strutt, and
T A Weaver
January 1998,
The International journal of developmental biology,
R A White, and
J J Brookman, and
A P Gould, and
L A Meadows, and
L S Shashidhara, and
D I Strutt, and
T A Weaver
November 1988,
Cell,
R A White, and
J J Brookman, and
A P Gould, and
L A Meadows, and
L S Shashidhara, and
D I Strutt, and
T A Weaver
September 2000,
Genetics,
R A White, and
J J Brookman, and
A P Gould, and
L A Meadows, and
L S Shashidhara, and
D I Strutt, and
T A Weaver
April 1993,
The EMBO journal,
R A White, and
J J Brookman, and
A P Gould, and
L A Meadows, and
L S Shashidhara, and
D I Strutt, and
T A Weaver
June 2010,
Genes to cells : devoted to molecular & cellular mechanisms,
R A White, and
J J Brookman, and
A P Gould, and
L A Meadows, and
L S Shashidhara, and
D I Strutt, and
T A Weaver
September 1986,
The EMBO journal,
