-transparent.png){kind=logo}
The aim of our study was to establish an efficient system for the in vitro production of the insect pathogenic Autographa californica nuclear polyhedrosis virus in a Spodoptera frugiperda cell line. We optimized cultivation conditions for cell proliferation as well as for virus replication in a 1.5 litre stirred tank bioreactor. Cell and virus propagation were found to be optimal at a constant oxygen tension of 40%. In order to provide sufficient nutrients during virus synthesis filtration and perfusion devices were connected to the bioreactor. A virus production procedure in a repeated batch mode by using a two stage bioreactor system is described. Stage I was optimized for cell production and stage II for virus production.
M Klöppinger, and
G Fertig, and
E Fraune, and
H G Miltenburger
August 1994,
Virology,
M Klöppinger, and
G Fertig, and
E Fraune, and
H G Miltenburger
June 1990,
Veterinary microbiology,
M Klöppinger, and
G Fertig, and
E Fraune, and
H G Miltenburger
June 1982,
Virology,
M Klöppinger, and
G Fertig, and
E Fraune, and
H G Miltenburger
February 1980,
Virology,
M Klöppinger, and
G Fertig, and
E Fraune, and
H G Miltenburger
January 1987,
Virology,
M Klöppinger, and
G Fertig, and
E Fraune, and
H G Miltenburger
March 1989,
Science (New York, N.Y.),
M Klöppinger, and
G Fertig, and
E Fraune, and
H G Miltenburger
December 1992,
The Journal of general virology,
M Klöppinger, and
G Fertig, and
E Fraune, and
H G Miltenburger
October 1996,
Journal of virology,
M Klöppinger, and
G Fertig, and
E Fraune, and
H G Miltenburger
June 1980,
Virology,
M Klöppinger, and
G Fertig, and
E Fraune, and
H G Miltenburger
March 1990,
Virology,
