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Generation of transgenic dairy cattle using 'in vitro' embryo production. 1991
P Krimpenfort, and
A Rademakers, and
W Eyestone, and
A van der Schans, and
S van den Broek, and
P Kooiman, and
E Kootwijk, and
G Platenburg, and
F Pieper, and
R Strijker
Department of Embryology, Gene Pharming Europe B.V., Leiden, The Netherlands.
We have combined gene transfer, by microinjection, with 'in vitro' embryo production technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected embryos that have been cultured from immature oocytes. Using this approach, we have established 21 pregnancies from which 19 calves were born. Southern blot analysis proved that in two cases the microinjected DNA had been integrated in the host genome.
Related Publications
P Krimpenfort, and
A Rademakers, and
W Eyestone, and
A van der Schans, and
S van den Broek, and
P Kooiman, and
E Kootwijk, and
G Platenburg, and
F Pieper, and
R Strijker
January 1999,
Theriogenology,
P Krimpenfort, and
A Rademakers, and
W Eyestone, and
A van der Schans, and
S van den Broek, and
P Kooiman, and
E Kootwijk, and
G Platenburg, and
F Pieper, and
R Strijker
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W Eyestone, and
A van der Schans, and
S van den Broek, and
P Kooiman, and
E Kootwijk, and
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R Strijker
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P Krimpenfort, and
A Rademakers, and
W Eyestone, and
A van der Schans, and
S van den Broek, and
P Kooiman, and
E Kootwijk, and
G Platenburg, and
F Pieper, and
R Strijker
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Bio/technology (Nature Publishing Company),
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A Rademakers, and
W Eyestone, and
A van der Schans, and
S van den Broek, and
P Kooiman, and
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F Pieper, and
R Strijker
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P Krimpenfort, and
A Rademakers, and
W Eyestone, and
A van der Schans, and
S van den Broek, and
P Kooiman, and
E Kootwijk, and
G Platenburg, and
F Pieper, and
R Strijker
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P Krimpenfort, and
A Rademakers, and
W Eyestone, and
A van der Schans, and
S van den Broek, and
P Kooiman, and
E Kootwijk, and
G Platenburg, and
F Pieper, and
R Strijker
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Theriogenology,
P Krimpenfort, and
A Rademakers, and
W Eyestone, and
A van der Schans, and
S van den Broek, and
P Kooiman, and
E Kootwijk, and
G Platenburg, and
F Pieper, and
R Strijker
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Theriogenology,
P Krimpenfort, and
A Rademakers, and
W Eyestone, and
A van der Schans, and
S van den Broek, and
P Kooiman, and
E Kootwijk, and
G Platenburg, and
F Pieper, and
R Strijker
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Reproduction in domestic animals = Zuchthygiene,
P Krimpenfort, and
A Rademakers, and
W Eyestone, and
A van der Schans, and
S van den Broek, and
P Kooiman, and
E Kootwijk, and
G Platenburg, and
F Pieper, and
R Strijker
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Research in veterinary science,
