BIOMAT Database Logo BIOMAT Database Logo

Monitoring of the production of monoclonal antibodies by hybridomas. Part I: Long-term cultivation in hollow fibre bioreactors using serum-free medium. 1991

R van Erp, and M Adorf, and A P van Sommeren, and O T Schönherr, and T C Gribnau
Clinical Lab Systems Research Unit, Organon Teknika B.V., Boxtel, The Netherlands.

The long-term cultivation of hybridoma cells in hollow fibre bioreactors using serum-free medium, was monitored with respect to quantitative and qualitative aspects of the produced mAbs, cell viability, LDH and proteolytic activity. During the culture periods of hybridoma cells producing mAb OT-1C and 3A, the mAb concentration showed a decreasing trend with a concomitant increase of IgG fragments. The major IgG fragments did not bind the antigen and the molecular weights were significantly different from the corresponding IgG heavy and light chains. In addition, a good correlation was found between cell lysis, the presence of acid protease(s) and IgG fragments. The physicochemical and immunochemical properties of the "intact" mAbs (such as molecular weights, IEF patterns and affinity) did not change significantly during the culture period.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007525 Isoelectric Focusing Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point. Electrofocusing,Focusing, Isoelectric
D007770 L-Lactate Dehydrogenase A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist. Lactate Dehydrogenase,Dehydrogenase, L-Lactate,Dehydrogenase, Lactate,L Lactate Dehydrogenase
D010450 Endopeptidases A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS. Endopeptidase,Peptide Peptidohydrolases
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D006063 Chorionic Gonadotropin A gonadotropic glycoprotein hormone produced primarily by the PLACENTA. Similar to the pituitary LUTEINIZING HORMONE in structure and function, chorionic gonadotropin is involved in maintaining the CORPUS LUTEUM during pregnancy. CG consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is virtually identical to the alpha subunits of the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity (CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN). Chorionic Gonadotropin, Human,HCG (Human Chorionic Gonadotropin),Biogonadil,Choriogonadotropin,Choriogonin,Chorulon,Gonabion,Human Chorionic Gonadotropin,Pregnyl,Gonadotropin, Chorionic,Gonadotropin, Human Chorionic
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006825 Hybridomas Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell. Hybridoma
D000371 Agglutination The clumping together of suspended material resulting from the action of AGGLUTININS.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

R van Erp, and M Adorf, and A P van Sommeren, and O T Schönherr, and T C Gribnau
Monoclonal antibody production in hollow fiber bioreactors using serum-free medium.
February 1989, BioTechniques,
R van Erp, and M Adorf, and A P van Sommeren, and O T Schönherr, and T C Gribnau
[Production of antibodies by hybridomas in a serum-free culture medium].
January 1984, Eksperimental'naia onkologiia,
R van Erp, and M Adorf, and A P van Sommeren, and O T Schönherr, and T C Gribnau
An integration of cultivation and purification in membrane bioreactors: production of monoclonal antibodies and lymphokines by microencapsulated hybridomas.
January 1988, Molecular biotherapy,
R van Erp, and M Adorf, and A P van Sommeren, and O T Schönherr, and T C Gribnau
Production of monoclonal antibodies in serum free medium.
January 1980, Journal of immunological methods,
R van Erp, and M Adorf, and A P van Sommeren, and O T Schönherr, and T C Gribnau
Long-term production of human monoclonal antibodies by five human-mouse heterohybridomas in a serum-free medium.
October 1998, Hybridoma,
R van Erp, and M Adorf, and A P van Sommeren, and O T Schönherr, and T C Gribnau
Large-scale production of murine monoclonal antibodies using hollow fiber bioreactors.
September 1988, BioTechniques,
R van Erp, and M Adorf, and A P van Sommeren, and O T Schönherr, and T C Gribnau
The use of oxygen carriers for increasing the production of monoclonal antibodies from hollow fibre bioreactors.
January 1998, Research in immunology,
R van Erp, and M Adorf, and A P van Sommeren, and O T Schönherr, and T C Gribnau
In vitro production of monoclonal antibodies under serum-free conditions using a compact and inexpensive hollow fibre cell culture unit.
July 1988, Journal of immunological methods,
R van Erp, and M Adorf, and A P van Sommeren, and O T Schönherr, and T C Gribnau
Antibody production by human X human hybridomas in serum-free medium.
April 1985, Journal of immunological methods,
R van Erp, and M Adorf, and A P van Sommeren, and O T Schönherr, and T C Gribnau
[Production of monoclonal antibodies in serum-free medium in dialysis tubing].
January 1991, Allergie und Immunologie,
Copied contents to your clipboard!