This study was undertaken to introduce new tentacle ion exchange materials to the biochromatographer. The differences in the bioseparation media have been shown with a four-component protein mixture. By controlling the sample loading and the flow rate, each material can be made to perform satisfactorily, according to the demands of the biochromatographer, whether for high resolution or high sample capacity. The controlled manufacturing techniques of the Fractogel and LiChrospher DEAE materials make any analytical or process separations equivalent and reproducible. When even higher capacities are required, the biochromatographer need only use the wider diameter Superformance glass columns packed with the equivalent Fractogel.