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A cDNA library corresponding to mite protein was screened using anti-Der f II, a major allergen from the house dust mite Dermatophagoides farinae, antibody. Three possible clones were obtained that contained cDNA fragments coding for Der f II, and the nucleotide sequences of the fragments were determined. There were minor differences observed affecting the deduced amino acid sequence among the three cDNA fragments. The amino acid sequence of the purified native Der f II protein could be analyzed to 45 residues from the N-terminus. As a result of comparison, all the three cDNA fragments code for a mature protein with a derived molecular weight of about 14,000. The amino acid sequence was not homologous to any known protein sequences and it contained six cysteine residues and no N-glycosylation sites.
T Yuuki, and
Y Okumura, and
T Ando, and
H Yamakawa, and
M Suko, and
M Haida, and
H Okudaira
May 2008,
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
T Yuuki, and
Y Okumura, and
T Ando, and
H Yamakawa, and
M Suko, and
M Haida, and
H Okudaira
January 1988,
International archives of allergy and applied immunology,
T Yuuki, and
Y Okumura, and
T Ando, and
H Yamakawa, and
M Suko, and
M Haida, and
H Okudaira
January 1991,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
T Yuuki, and
Y Okumura, and
T Ando, and
H Yamakawa, and
M Suko, and
M Haida, and
H Okudaira
January 1991,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
T Yuuki, and
Y Okumura, and
T Ando, and
H Yamakawa, and
M Suko, and
M Haida, and
H Okudaira
October 2008,
Journal of bioscience and bioengineering,
T Yuuki, and
Y Okumura, and
T Ando, and
H Yamakawa, and
M Suko, and
M Haida, and
H Okudaira
May 2016,
Protein expression and purification,
T Yuuki, and
Y Okumura, and
T Ando, and
H Yamakawa, and
M Suko, and
M Haida, and
H Okudaira
October 1995,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
T Yuuki, and
Y Okumura, and
T Ando, and
H Yamakawa, and
M Suko, and
M Haida, and
H Okudaira
February 2001,
Veterinary immunology and immunopathology,
T Yuuki, and
Y Okumura, and
T Ando, and
H Yamakawa, and
M Suko, and
M Haida, and
H Okudaira
August 1993,
Journal of immunology (Baltimore, Md. : 1950),
T Yuuki, and
Y Okumura, and
T Ando, and
H Yamakawa, and
M Suko, and
M Haida, and
H Okudaira
January 1988,
The Journal of experimental medicine,
