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To enzymatically synthesize active metabolites of vitamin D3, we screened about 500 bacterial strains and 450 fungal strains, of which 12 strains were able to convert vitamin D3 to 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3] via 25-hydroxyvitamin D3 [25(OH)D3]. The conversion activity was only detected in strains belonging to the genus Amycolata among all the organisms tested. A preparative-scale conversion of vitamin D3 to 25(OH)D3 and 1 alpha,25(OH)2D3 in a 200-1 tank fermentor using A. autotrophica FERM BP-1573 was accomplished, yielding 8.3 mg 25(OH)D3/l culture and 0.17 mg 1 alpha,25(OH)2D3/l culture. A related compound, vitamin D2, could be also converted to 25-hydroxyvitamin D2 and 1 alpha,25-dihydroxyvitamin D2 using the same strain. The cytochrome P-450 of FERM BP-1573 was detected by reduced CO difference spectra in whole-cell suspensions. Vitamin D3 in the culture induced cytochrome P-450 and the conversion activity simultaneously, suggesting that the hydroxylation at C-25 of vitamin D3 and at C-1 of 25(OH)D3 originates from cytochrome P-450.
J Sasaki, and
A Miyazaki, and
M Saito, and
T Adachi, and
K Mizoue, and
K Hanada, and
S Omura
October 1991,
Applied and environmental microbiology,
J Sasaki, and
A Miyazaki, and
M Saito, and
T Adachi, and
K Mizoue, and
K Hanada, and
S Omura
March 1983,
Biochemistry,
J Sasaki, and
A Miyazaki, and
M Saito, and
T Adachi, and
K Mizoue, and
K Hanada, and
S Omura
January 1995,
Bone,
J Sasaki, and
A Miyazaki, and
M Saito, and
T Adachi, and
K Mizoue, and
K Hanada, and
S Omura
February 1995,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
J Sasaki, and
A Miyazaki, and
M Saito, and
T Adachi, and
K Mizoue, and
K Hanada, and
S Omura
November 1989,
Journal of molecular endocrinology,
J Sasaki, and
A Miyazaki, and
M Saito, and
T Adachi, and
K Mizoue, and
K Hanada, and
S Omura
July 1976,
The Journal of clinical investigation,
J Sasaki, and
A Miyazaki, and
M Saito, and
T Adachi, and
K Mizoue, and
K Hanada, and
S Omura
September 1987,
Bone and mineral,
J Sasaki, and
A Miyazaki, and
M Saito, and
T Adachi, and
K Mizoue, and
K Hanada, and
S Omura
January 1980,
Calcified tissue international,
J Sasaki, and
A Miyazaki, and
M Saito, and
T Adachi, and
K Mizoue, and
K Hanada, and
S Omura
November 2003,
Blood,
J Sasaki, and
A Miyazaki, and
M Saito, and
T Adachi, and
K Mizoue, and
K Hanada, and
S Omura
February 1991,
Clinical nephrology,
