Biomaterial Database

L-lysine repression of penicillin biosynthesis and the expression of penicillin biosynthesis genes acvA and ipnA in Aspergillus nidulans. 1992

A A Brakhage, and G Turner

Department of Molecular Biology and Biotechnology, University of Sheffield, UK.

The addition of 0.1 M L-lysine to the fermentation medium reduced the production of penicillin by about 50% in Aspergillus nidulans. To analyse this effect at the molecular level, the expression of the penicillin biosynthesis genes acvA and ipnA, encoding delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase and isopenicillin N synthetase, was studied by using translational fusions with different reporter genes (strain AXB4A, acvA-uidA, ipnA-lacZ fusions; AXB4B, acvA-lacZ, ipnA-uidA fusions) integrated in single copy at the chromosomal argB locus of Aspergillus nidulans. Irrespective of the reporter genes used the expression of acvA and ipnA fusion genes was repressed in L-lysine grown cultures. The expression of a fusion gene of an A. nidulans primary metabolism gene (oliC-lacZ) was not affected by L-lysine.

UI	MeSH Term	Description	Entries
D008239	Lysine	An essential amino acid. It is often added to animal feed.	Enisyl,L-Lysine,Lysine Acetate,Lysine Hydrochloride,Acetate, Lysine,L Lysine
D010088	Oxidoreductases	The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)	Dehydrogenases,Oxidases,Oxidoreductase,Reductases,Dehydrogenase,Oxidase,Reductase
D010404	Penicillin V	A broad-spectrum penicillin antibiotic used orally in the treatment of mild to moderate infections by susceptible gram-positive organisms.	Penicillin, Phenoxymethyl,Apocillin,Beromycin,Beromycin, Penicillin,Berromycin, Penicillin,Betapen,Fenoxymethylpenicillin,Pen VK,Penicillin V Potassium,Penicillin V Sodium,Penicillin VK,Phenoxymethylpenicillin,V-Cillin K,Vegacillin,Penicillin Beromycin,Penicillin Berromycin,Phenoxymethyl Penicillin,Potassium, Penicillin V,Sodium, Penicillin V,V Cillin K,V Sodium, Penicillin,VCillin K
D010453	Peptide Synthases	Ligases that catalyze the joining of adjacent AMINO ACIDS by the formation of carbon-nitrogen bonds between their carboxylic acid groups and amine groups.	Peptide Synthetases,Acid-Amino-Acid Ligases,Acid Amino Acid Ligases,Ligases, Acid-Amino-Acid,Synthases, Peptide,Synthetases, Peptide
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D005285	Fermentation	Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.	Fermentations
D005800	Genes, Fungal	The functional hereditary units of FUNGI.	Fungal Genes,Fungal Gene,Gene, Fungal
D001233	Aspergillus nidulans	A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.	Aspergillus nidulellus,Emericella nidulans
D015074	2-Aminoadipic Acid	A metabolite in the principal biochemical pathway of lysine. It antagonizes neuroexcitatory activity modulated by the glutamate receptor, N-METHYL-D-ASPARTATE; (NMDA).	alpha-Aminoadipic Acid,2 Aminoadipic Acid,2-Aminohexanedioic Acid,2 Aminohexanedioic Acid,Acid, 2 Aminoadipic,Acid, 2-Aminoadipic,Acid, 2-Aminohexanedioic,Acid, alpha-Aminoadipic,Aminoadipic Acid, 2,alpha Aminoadipic Acid
D015966	Gene Expression Regulation, Fungal	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.	Fungal Gene Expression Regulation,Regulation of Gene Expression, Fungal,Regulation, Gene Expression, Fungal