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Menadione (MD; 2-methyl-1,4-naphthoquinone), a redox cycling quinone was shown to induce single (ss)- and double (ds)-strand DNA breaks in human MCF-7 cells. This DNA damage was mediated via the hydroxyl radical as evidenced by electron spin resonance spectroscopy (ESR) studies utilizing the spin trap, 5,5-dimethyl-1-pyrroline-1-oxide. The free radical production and DNA damage were shown to play a role in MD cytotoxicity as revealed by the reversal of MD toxicity and inhibition of hydroxyl radical production by exogenously added catalase. The role of NADPH quinone acceptor oxidoreductase in the metabolism of MD was evaluated. Purified quinone acceptor oxidoreductase in combination with MD resulted in the production of significant levels of the hydroxyl radical as measured by ESR. Dicumarol, an inhibitor of quinone acceptor oxidoreductase, decreased the production of the hydroxyl radical and attenuated DNA strand breaks in MCF-7 cells treated with MD.
L M Nutter, and
E O Ngo, and
G R Fisher, and
P L Gutierrez
January 1991,
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September 1993,
Chemico-biological interactions,
L M Nutter, and
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P L Gutierrez
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L M Nutter, and
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P L Gutierrez
November 1990,
Carcinogenesis,
L M Nutter, and
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P L Gutierrez
August 1992,
Photochemistry and photobiology,
L M Nutter, and
E O Ngo, and
G R Fisher, and
P L Gutierrez
November 1990,
Cancer research,
L M Nutter, and
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G R Fisher, and
P L Gutierrez
March 1977,
Biochimica et biophysica acta,
L M Nutter, and
E O Ngo, and
G R Fisher, and
P L Gutierrez
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Cancer research,
L M Nutter, and
E O Ngo, and
G R Fisher, and
P L Gutierrez
January 2021,
Oxidative medicine and cellular longevity,
L M Nutter, and
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G R Fisher, and
P L Gutierrez
May 1992,
Toxicology and applied pharmacology,
