BIOMAT Database Logo BIOMAT Database Logo

Concomitant expression of receptor subtype and isopeptide of endothelin by human adrenal gland. 1992

T Imai, and Y Hirata, and S Eguchi, and K Kanno, and K Ohta, and T Emori, and A Sakamoto, and M Yanagisawa, and T Masaki, and F Marumo
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.

We studied whether specific receptors for endothelin (ET) isopeptide exist in human aldosterone-producing adenoma and normal adrenal cortex, and whether ET isopeptides are produced by human adrenal gland. Competitive binding studies using [125I]ET-1 as a radioligand revealed the presence of a single class of high-affinity binding sites for ET-1 with the apparent KD of 70 +/- 31 pM and Bmax of 226 +/- 139 fmol/mg protein in adenoma membranes almost comparable to those in adjacent normal cortex. The apparent Ki for ET-2 and ET-3 were 89 +/- 33 pM and 82 +/- 16 pM, respectively. Northern blot analysis of poly(A)+ RNA of adenoma and adjacent normal cortex using cDNAs for ET receptor subtype (ETA, ETB) and ET isopeptide (ET-1, ET-3) as probes revealed that ETA and ETB receptors as well as ET isopeptides (preproET-1, preproET-3) are concomitantly expressed in both tissues. Our data demonstrate for the first time that ET receptor subtype (ETA and ETB) and ET isopeptide (ET-1 and ET-3) are concomitantly expressed by human adrenal cortex, suggesting the potential role of ETs as a local mediator in human adrenal gland.

UI MeSH Term Description Entries
D007536 Isomerism The phenomenon whereby certain chemical compounds have structures that are different although the compounds possess the same elemental composition. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed) Isomerisms
D007700 Kinetics The rate dynamics in chemical or physical systems.
D011061 Poly A A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties. Adenine Polynucleotides,Polyadenylic Acids,Poly(rA),Polynucleotides, Adenine
D011956 Receptors, Cell Surface Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands. Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000236 Adenoma A benign epithelial tumor with a glandular organization. Adenoma, Basal Cell,Adenoma, Follicular,Adenoma, Microcystic,Adenoma, Monomorphic,Adenoma, Papillary,Adenoma, Trabecular,Adenomas,Adenomas, Basal Cell,Adenomas, Follicular,Adenomas, Microcystic,Adenomas, Monomorphic,Adenomas, Papillary,Adenomas, Trabecular,Basal Cell Adenoma,Basal Cell Adenomas,Follicular Adenoma,Follicular Adenomas,Microcystic Adenoma,Microcystic Adenomas,Monomorphic Adenoma,Monomorphic Adenomas,Papillary Adenoma,Papillary Adenomas,Trabecular Adenoma,Trabecular Adenomas
D000302 Adrenal Cortex The outer layer of the adrenal gland. It is derived from MESODERM and comprised of three zones (outer ZONA GLOMERULOSA, middle ZONA FASCICULATA, and inner ZONA RETICULARIS) with each producing various steroids preferentially, such as ALDOSTERONE; HYDROCORTISONE; DEHYDROEPIANDROSTERONE; and ANDROSTENEDIONE. Adrenal cortex function is regulated by pituitary ADRENOCORTICOTROPIN. Cortex, Adrenal
D000306 Adrenal Cortex Neoplasms Tumors or cancers of the ADRENAL CORTEX. Adrenocortical Cancer,Cancer of Adrenal Cortex,Adrenal Cortex Cancer,Cancer of the Adrenal Cortex,Neoplasms, Adrenal Cortex,Adrenal Cortex Cancers,Adrenal Cortex Neoplasm,Adrenocortical Cancers,Cancer, Adrenal Cortex,Cancer, Adrenocortical,Cancers, Adrenal Cortex,Cancers, Adrenocortical,Neoplasm, Adrenal Cortex
D000311 Adrenal Glands A pair of glands located at the cranial pole of each of the two KIDNEYS. Each adrenal gland is composed of two distinct endocrine tissues with separate embryonic origins, the ADRENAL CORTEX producing STEROIDS and the ADRENAL MEDULLA producing NEUROTRANSMITTERS. Adrenal Gland,Gland, Adrenal,Glands, Adrenal

Related Publications

T Imai, and Y Hirata, and S Eguchi, and K Kanno, and K Ohta, and T Emori, and A Sakamoto, and M Yanagisawa, and T Masaki, and F Marumo
Cloning and expression of a human endothelin receptor: subtype A.
October 1992, The American journal of the medical sciences,
T Imai, and Y Hirata, and S Eguchi, and K Kanno, and K Ohta, and T Emori, and A Sakamoto, and M Yanagisawa, and T Masaki, and F Marumo
Cloning of a cDNA encoding a non-isopeptide-selective subtype of the endothelin receptor.
January 1990, Nature,
T Imai, and Y Hirata, and S Eguchi, and K Kanno, and K Ohta, and T Emori, and A Sakamoto, and M Yanagisawa, and T Masaki, and F Marumo
Molecular cloning of a non-isopeptide-selective human endothelin receptor.
July 1991, Biochemical and biophysical research communications,
T Imai, and Y Hirata, and S Eguchi, and K Kanno, and K Ohta, and T Emori, and A Sakamoto, and M Yanagisawa, and T Masaki, and F Marumo
Regulation of ANG II-receptor subtype and its gene expression in adrenal gland.
August 1996, The American journal of physiology,
T Imai, and Y Hirata, and S Eguchi, and K Kanno, and K Ohta, and T Emori, and A Sakamoto, and M Yanagisawa, and T Masaki, and F Marumo
Dopamine receptor expression and function in human normal adrenal gland and adrenal tumors.
September 2004, The Journal of clinical endocrinology and metabolism,
T Imai, and Y Hirata, and S Eguchi, and K Kanno, and K Ohta, and T Emori, and A Sakamoto, and M Yanagisawa, and T Masaki, and F Marumo
[Endothelin-1 receptors of the normal adrenal gland and adrenal tumors in human].
July 1996, Zhonghua nei ke za zhi,
T Imai, and Y Hirata, and S Eguchi, and K Kanno, and K Ohta, and T Emori, and A Sakamoto, and M Yanagisawa, and T Masaki, and F Marumo
Endothelin-1 and the adrenal gland.
January 2000, Journal of cardiovascular pharmacology,
T Imai, and Y Hirata, and S Eguchi, and K Kanno, and K Ohta, and T Emori, and A Sakamoto, and M Yanagisawa, and T Masaki, and F Marumo
Cloning and expression of an endothelin receptor subtype B from human prostate that mediates contraction.
April 1995, Molecular pharmacology,
T Imai, and Y Hirata, and S Eguchi, and K Kanno, and K Ohta, and T Emori, and A Sakamoto, and M Yanagisawa, and T Masaki, and F Marumo
Differential distribution of endothelin peptides and receptors in human adrenal gland.
November 1996, The Histochemical journal,
T Imai, and Y Hirata, and S Eguchi, and K Kanno, and K Ohta, and T Emori, and A Sakamoto, and M Yanagisawa, and T Masaki, and F Marumo
Ligand binding domain of the human endothelin-B subtype receptor.
June 1995, Protein expression and purification,
Copied contents to your clipboard!