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Epstein-Barr (EBV)-immortalized B cell clones were established from CD5+ and CD5- cord blood B cells separated by flow cytometry. We have previously shown that IgM from many of the clones was polyreactive, exhibiting reactivity with a number of autoantigens. In this study, IgM produced by the clones was analysed by MoAb for the expression of cross-reactive idiotypes (CRI) associated with rheumatoid factor paraproteins and from defined VH and V kappa subgroups of immunoglobulin heavy and light chains. IgM produced by clones established from CD5+ and CD5- B cells expressed the VH I associated idiotope G8. Furthermore, IgM produced by both sets of clones exhibited a similar frequency of VH III heavy chain subgroup expression, as determined by reactivity with staphylococcal protein A (SpA) and VH III-associated CRI expression (B6 and/or D12). In contrast, expression of the V kappa III-associated 17.109 CRI was significantly higher in IgM antibodies produced by clones established from CD5+ compared with the CD5- clones (32 versus 5%: P less than 0.05). Analysis of the VH and VL subgroup expression by IgM produced by the CD5+ and CD5- cord blood clones, and their autoantigen reactivity profile did not reveal restriction or selection within CD5+ and CD5- populations. However, our data suggest that differences may exist in the expression of certain germ-line genes between CD5+ and CD5- cord blood B cells and might indicate an expansion of CD5+ B cells within the fetal environment.
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R A Mageed, and
P Y Youinou, and
B Yuksel, and
R Jefferis, and
P M Lydyard
November 1995,
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L E Mackenzie, and
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P M Lydyard
November 1991,
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L E Mackenzie, and
R A Mageed, and
P Y Youinou, and
B Yuksel, and
R Jefferis, and
P M Lydyard
November 2006,
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L E Mackenzie, and
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B Yuksel, and
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P M Lydyard
February 1997,
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L E Mackenzie, and
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B Yuksel, and
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P M Lydyard
May 1997,
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L E Mackenzie, and
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B Yuksel, and
R Jefferis, and
P M Lydyard
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L E Mackenzie, and
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L E Mackenzie, and
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P M Lydyard
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L E Mackenzie, and
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P Y Youinou, and
B Yuksel, and
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P M Lydyard
November 1989,
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L E Mackenzie, and
R A Mageed, and
P Y Youinou, and
B Yuksel, and
R Jefferis, and
P M Lydyard
May 1991,
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