Levels of amylase, trypsin, chymotrypsin, and lipase in the pancreas and small intestinal chyme were measured in females from four lines of chickens. Two of the lines had undergone 32 generations of divergent selection for 56-day body weight, and in the other two lines selection for high or low weight had been relaxed for 5 generations. The diet used in the present experiment was that under which selection had been practiced (20% crude protein and 2,685 kcal of ME/kg). Comparisons between divergently selected lines at common ages revealed higher enzyme levels for high- than low-weight lines. When comparisons were made at a common body weight (80 +/- 5 g) there were no differences between lines. These results suggested that correlated responses in feed intake were mediating the regulation of digestive enzyme levels in the pancreas and in intestinal chyme of growth-selected lines of chickens. Chicks from high-weight lines had elevated enzyme levels after a mild feed restriction compared with those provided ad libitum access to feed. It was hypothesized that hyperphagia associated with the high-weight lines in combination with a mild feed restriction and the associated meal feeding stimulated synthesis and secretion of digestive enzymes.