Isolation and characterization of cDNA clones encoding cdc2 homologues from Oryza sativa: a functional homologue and cognate variants. 1992

J Hashimoto, and T Hirabayashi, and Y Hayano, and S Hata, and Y Ohashi, and I Suzuka, and T Utsugi, and A Toh-e, and Y Kikuchi
National Institute of Agrobiological Resources, Ibaraki, Japan.

Using probes obtained by PCR amplification, we have isolated two cognate rice cDNAs (cdc2Os-1 and cdc2Os-2) encoding structural homologues of the cdc2+/CDC28 (cdc2) protein kinase from a cDNA library prepared from cultured rice cells. Comparison of the deduced amino acid sequences of cdc2Os-1 and cdc2Os-2 showed that they are 83% identical. They are 62% identical to CDC28 of Saccharomyces cerevisiae and much more similar to the yeast and mammalian p34cdc2 kinases than to rice R2, a cdc2-related kinase isolated previously by screening the same rice cDNA library with a different oligonucleotide probe. Southern blot analysis indicated that the three rice clones (cdc2Os-1, cdc2Os-2 and R2) are derived from distinct genes and are each found in a single copy per rice haploid genome. RNA blot analysis revealed that these genes are expressed in proliferating rice cells and in young rice seedlings. cdc2Os-1 could complement a temperature-sensitive yeast mutant of cdc28. However, despite the similarity in structure, both cdc2Os-2 and R2 were unable to complement the same mutant. Thus, the present results demonstrate the presence of structurally related, but functionally distinct cognates of the cdc2 cell cycle kinase in rice.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D005816 Genetic Complementation Test A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell. Allelism Test,Cis Test,Cis-Trans Test,Complementation Test,Trans Test,Allelism Tests,Cis Tests,Cis Trans Test,Cis-Trans Tests,Complementation Test, Genetic,Complementation Tests,Complementation Tests, Genetic,Genetic Complementation Tests,Trans Tests
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012275 Oryza A genus of grass family (Poaceae) that include several rice species. Oryza sativa,Rice,Rices
D012313 RNA A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed) RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated
D012689 Sequence Homology, Nucleic Acid The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function. Base Sequence Homology,Homologous Sequences, Nucleic Acid,Homologs, Nucleic Acid Sequence,Homology, Base Sequence,Homology, Nucleic Acid Sequence,Nucleic Acid Sequence Homologs,Nucleic Acid Sequence Homology,Sequence Homology, Base,Base Sequence Homologies,Homologies, Base Sequence,Sequence Homologies, Base

Related Publications

J Hashimoto, and T Hirabayashi, and Y Hayano, and S Hata, and Y Ohashi, and I Suzuka, and T Utsugi, and A Toh-e, and Y Kikuchi
April 1991, Proceedings of the National Academy of Sciences of the United States of America,
J Hashimoto, and T Hirabayashi, and Y Hayano, and S Hata, and Y Ohashi, and I Suzuka, and T Utsugi, and A Toh-e, and Y Kikuchi
August 2007, Biotechnology letters,
J Hashimoto, and T Hirabayashi, and Y Hayano, and S Hata, and Y Ohashi, and I Suzuka, and T Utsugi, and A Toh-e, and Y Kikuchi
October 1989, Current eye research,
J Hashimoto, and T Hirabayashi, and Y Hayano, and S Hata, and Y Ohashi, and I Suzuka, and T Utsugi, and A Toh-e, and Y Kikuchi
March 1993, The Journal of biological chemistry,
J Hashimoto, and T Hirabayashi, and Y Hayano, and S Hata, and Y Ohashi, and I Suzuka, and T Utsugi, and A Toh-e, and Y Kikuchi
May 1995, The Biochemical journal,
J Hashimoto, and T Hirabayashi, and Y Hayano, and S Hata, and Y Ohashi, and I Suzuka, and T Utsugi, and A Toh-e, and Y Kikuchi
September 2005, Journal of biochemistry and molecular biology,
J Hashimoto, and T Hirabayashi, and Y Hayano, and S Hata, and Y Ohashi, and I Suzuka, and T Utsugi, and A Toh-e, and Y Kikuchi
January 1996, DNA sequence : the journal of DNA sequencing and mapping,
J Hashimoto, and T Hirabayashi, and Y Hayano, and S Hata, and Y Ohashi, and I Suzuka, and T Utsugi, and A Toh-e, and Y Kikuchi
October 1996, DNA research : an international journal for rapid publication of reports on genes and genomes,
J Hashimoto, and T Hirabayashi, and Y Hayano, and S Hata, and Y Ohashi, and I Suzuka, and T Utsugi, and A Toh-e, and Y Kikuchi
March 1992, Plant molecular biology,
J Hashimoto, and T Hirabayashi, and Y Hayano, and S Hata, and Y Ohashi, and I Suzuka, and T Utsugi, and A Toh-e, and Y Kikuchi
November 1992, The Plant journal : for cell and molecular biology,
Copied contents to your clipboard!