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Using immunohistochemical techniques and mAb MT2, we describe here a novel extracellular matrix (ECM) molecule that is developmentally regulated during limb regeneration in adult newts. The MT2 antigen appears during preblastema stages, is most abundant during blastema stages, and persists, near undifferentiated cells, until digit stages. The MT2 antigen is located in an acellular layer under the wound epithelium and throughout the ECM of the undifferentiated mesenchyme as a thick, cord-like component. In unamputated limbs mAb MT2 reactivity is restricted to tendons, myotendinous junctions, periosteum and to a layer of material beneath the epidermis. In both unamputated limbs and regenerating limbs, the reactivity to mAb MT2 colocalizes closely with urodele tenascin. Immunoblot analysis of blastema extracts showed that the unreduced form of the MT2 antigen is a large, polydispersed protein of approximately the same size as tenascin. However, based upon (a) molecular weights of reduced subunits, (b) competition experiments on tissue sections, and (c) analysis of molecules immunoprecipitated by mAb MT2, we conclude that the MT2 substance is unrelated biochemically to tenascin. The results from immunoblots, enzyme digestions and DEAE-Sephacell binding studies suggest that the unreduced MT2 antigen is a large protein composed of subunits which are connected by disulfide bonds. Reduction of the MT2 antigen results in three components recognized by mAb MT2. The largest of these reduced components is a chondroitin sulfate-like glycoprotein with a molecular weight (Mr) of 310-325 x 10(3). A second component (Mr, 285-300 x 10(3)) is the core protein of the 310-325 x 10(3) glycoprotein.(ABSTRACT TRUNCATED AT 250 WORDS)
K P Klatt, and
E V Yang, and
R A Tassava
October 2010,
Molecular therapy : the journal of the American Society of Gene Therapy,
K P Klatt, and
E V Yang, and
R A Tassava
January 2021,
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K P Klatt, and
E V Yang, and
R A Tassava
November 1991,
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K P Klatt, and
E V Yang, and
R A Tassava
December 1992,
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K P Klatt, and
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R A Tassava
April 1990,
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K P Klatt, and
E V Yang, and
R A Tassava
January 1996,
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K P Klatt, and
E V Yang, and
R A Tassava
March 2005,
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K P Klatt, and
E V Yang, and
R A Tassava
January 1992,
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K P Klatt, and
E V Yang, and
R A Tassava
June 1992,
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K P Klatt, and
E V Yang, and
R A Tassava
April 1986,
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