Biomaterial Database

Synergistic effects of murine stem cell factor in combination with a variety of cytokines on the expansion of murine hematopoietic progenitor cells in short-term suspension cultures. 1992

M Han

Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.

In the present study, it is represented that the ability of murine stem cell factor (SCF) to expand hematopoietic progenitor cells in short-term suspension culture when used alone or with IL-1 beta, IL-3, IL-6, M-CSF and IL1 beta Plus IL-3. SCF alone had a limited effect on the expansion of early primitive hematopoietic progenitor cells (CFU-HPP: high proliferative potential colony forming unit, and CFU-S: colony forming unit in spleen) even at a high concentration, but expanded mature hematopoietic progenitor cells (CFU-GM: colony forming unit-granulocyte/macrophage, and BFU-E: burst forming unit-erythroid) markedly at low concentrations. When SCF was used in combination with other cytokines, the expansion of primitive hematopoietic progenitor cells was significantly increased; namely, CFU-HPP were expanded approximately 2 to 5-fold compared with SCF alone. A marked expansion of hematopoietic progenitor cells was observed in a combination of SCF plus IL-1 beta plus IL-3. In this setting, CFU-S was increased 2.2-fold compared with the number of CFU-S in fresh bone marrow and CFU-HPP were increased 8.5-fold compared with the number of primary CFU-HPP. These results suggest that these factors may be utilized in experiments of murine bone marrow transplantation (BMT) and also in human BMT. Namely, the adequate number of hematopoiesic progenitor cells and stem cells required for the successful engraftment can be obtained from small volume of peripheral or bone marrow blood by this procedure, thus obtiating the donor's burden.

UI	MeSH Term	Description	Entries
D002455	Cell Division	The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.	M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004357	Drug Synergism	The action of a drug in promoting or enhancing the effectiveness of another drug.	Drug Potentiation,Drug Augmentation,Augmentation, Drug,Augmentations, Drug,Drug Augmentations,Drug Potentiations,Drug Synergisms,Potentiation, Drug,Potentiations, Drug,Synergism, Drug,Synergisms, Drug
D006412	Hematopoietic Stem Cells	Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.	Colony-Forming Units, Hematopoietic,Progenitor Cells, Hematopoietic,Stem Cells, Hematopoietic,Hematopoietic Progenitor Cells,Cell, Hematopoietic Progenitor,Cell, Hematopoietic Stem,Cells, Hematopoietic Progenitor,Cells, Hematopoietic Stem,Colony Forming Units, Hematopoietic,Colony-Forming Unit, Hematopoietic,Hematopoietic Colony-Forming Unit,Hematopoietic Colony-Forming Units,Hematopoietic Progenitor Cell,Hematopoietic Stem Cell,Progenitor Cell, Hematopoietic,Stem Cell, Hematopoietic,Unit, Hematopoietic Colony-Forming,Units, Hematopoietic Colony-Forming
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D016207	Cytokines	Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.	Cytokine
D016298	Hematopoietic Cell Growth Factors	These growth factors comprise a family of hematopoietic regulators with biological specificities defined by their ability to support proliferation and differentiation of blood cells of different lineages. ERYTHROPOIETIN and the COLONY-STIMULATING FACTORS belong to this family. Some of these factors have been studied and used in the treatment of chemotherapy-induced neutropenia, myelodysplastic syndromes, and bone marrow failure syndromes.	Hematopoietins,Hematopoietic Cell Growth Factor,Hematopoietic Stem Cell Stimulators,Hematopoietic Stem Cell-Activating Factors,Hematopoietic-CGF,Hematopoietic CGF
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D019089	Stem Cell Factor	A hematopoietic growth factor and the ligand of the cell surface c-kit protein (PROTO-ONCOGENE PROTEINS C-KIT). It is expressed during embryogenesis and is a growth factor for a number of cell types including the MAST CELLS and the MELANOCYTES in addition to the HEMATOPOIETIC STEM CELLS.	Mast Cell Growth Factor,Steel Factor,c-kit Ligand,kit Ligand,c kit Ligand