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Genetic analysis of an electrophoretic variant of the mitochondrial leucyl-tRNA synthetase [L-leucine:tRNALeu ligase (AMP-forming), EC 6.1.1.4] indicates that it is either an allele of or linked closely to leu-5ts, a mutant that is known to produce a cytoplasmic leucyl-tRNA synthetase with an altered affinity for leucine as well as being deficient in the production of the mitochondrial enzyme. Immunological analysis indicates that the two synthetases have little, if any, structural homology. The pattern of synthesis of the enzymes in leu-5ts revertants, the reciprocal relationship of the production of the two enzymes in response to a negative regulatory element, presumably of mitochondrial origin, as well as the lack of detectable structural homology, led to the proposal that the phenotype of leu-5ts results from a mutational alteration in the structural gene for the cytoplasmic enzyme in a region involved in the initiation of transcription of the adjacent gene for the mitochondrial enzyme.
P M Beauchamp, and
E W Horn, and
S R Gross
November 1989,
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P M Beauchamp, and
E W Horn, and
S R Gross
October 1980,
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P M Beauchamp, and
E W Horn, and
S R Gross
April 1973,
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P M Beauchamp, and
E W Horn, and
S R Gross
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P M Beauchamp, and
E W Horn, and
S R Gross
November 1975,
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P M Beauchamp, and
E W Horn, and
S R Gross
February 1981,
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P M Beauchamp, and
E W Horn, and
S R Gross
January 1988,
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P M Beauchamp, and
E W Horn, and
S R Gross
January 1980,
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P M Beauchamp, and
E W Horn, and
S R Gross
July 1995,
The EMBO journal,
P M Beauchamp, and
E W Horn, and
S R Gross
December 1978,
Archives of biochemistry and biophysics,
