Biomaterial Database

[Fluorometric determination of glibornuride in plasma and serum using 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole]. 1977

R Becker

Glibornuride, tolbutamide and gilbenclamide react on heating with 7-chloro-4-nitrobenzo-2-oxa-1.3-diazole (NBD-chloride) in amylacetate to form a highly fluorescent NBD-amine. A fluorometric determination of glibornuride is presented. Reaction conditions and the extraction of glibornuride from plasma and serum are described. The method is sensitive to measure drug levels after therapeutic doses. The detection limit is ca. 40 ng/ml and the recovery rate is ca. 100%. The coefficients of variation for the determination of drug levels of 1 to 0.1 mug/ml range from 4.1% to 12.1%.

UI	MeSH Term	Description	Entries
D009327	4-Chloro-7-nitrobenzofurazan	A benzofuran derivative used as a protein reagent since the terminal N-NBD-protein conjugate possesses interesting fluorescence and spectral properties. It has also been used as a covalent inhibitor of both beef heart mitochondrial ATPase and bacterial ATPase.	Chloronitrobenzoxadiazole,NBD Chloride,7-Chloro-4-nitrobenzofurazan,NBF-Cl,Nitrobenzoxadiazole Chloride,4 Chloro 7 nitrobenzofurazan,7 Chloro 4 nitrobenzofurazan,Chloride, NBD,Chloride, Nitrobenzoxadiazole,NBF Cl
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013453	Sulfonylurea Compounds	A class of compounds in which a sulfone functional group is attached to UREA.	Compounds, Sulfonylurea