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T-cell-enriched lymphocyte populations derived from the malignant exudate of a patient with ovarian carcinoma were exposed to autologous tumor cells in the mixed lymphocyte-tumor-cell culture (MLTC) and propagated for 42 days. Proliferation of lymphocytes depended on exposures to autologous tumor cells and on the presence of IL-2. After 7 days, the MLTC-lymphocytes lysed K562 and the autologous tumor cells. The latter effect was not inhibited by monoclonal antibodies (MAbs) reactive with MHC class-I antigens or with CD3. After 7 restimulations, the culture was enriched in CD8+ cells (92%) and showed selective lytic activity against the autologous tumor. This function was inhibited by the alpha-class I or alpha-CD3 MAbs, and also by antibodies reactive with the HLA B locus or B5 allele products. The antibodies reactive with HLA A molecules had no such effect. It seems therefore that the function of the CTLs was restricted by HLA B5. Analysis of the TCR beta genes indicated clonal T-cell expansion in this culture. This MLTC was 1 of 21 initiated with 11 blood- and 10 tumor-derived lymphocyte (TIL) populations prepared from the malignant effusions of ovarian carcinoma patients. None of these ex-vivo lymphocytes lysed autologous tumor cells. In 17 MLTCs the lymphocytes did not proliferate, and in 3 cultures the proliferation was maintained only for 2-3 weeks. In 3 of 4 cultures auto-tumor cytotoxicity was induced.
P Wang, and
Z s Végh, and
F Vánky, and
E Klein
July 1992,
International journal of cancer,
P Wang, and
Z s Végh, and
F Vánky, and
E Klein
March 1980,
Journal of immunology (Baltimore, Md. : 1950),
P Wang, and
Z s Végh, and
F Vánky, and
E Klein
January 2007,
Current HIV research,
P Wang, and
Z s Végh, and
F Vánky, and
E Klein
August 2001,
Biochemical and biophysical research communications,
P Wang, and
Z s Végh, and
F Vánky, and
E Klein
January 1988,
Cancer immunology, immunotherapy : CII,
P Wang, and
Z s Végh, and
F Vánky, and
E Klein
April 1988,
Transplantation proceedings,
P Wang, and
Z s Végh, and
F Vánky, and
E Klein
October 1981,
Lancet (London, England),
P Wang, and
Z s Végh, and
F Vánky, and
E Klein
June 2002,
Clinical cancer research : an official journal of the American Association for Cancer Research,
P Wang, and
Z s Végh, and
F Vánky, and
E Klein
December 1997,
AIDS (London, England),
P Wang, and
Z s Végh, and
F Vánky, and
E Klein
December 1977,
The Journal of experimental medicine,
