These studies were conducted to examine the influence of several variables on the growth hormone binding protein (GHBP) activity in serum of pigs. Continuous long-term porcine somatotropin (pST) injections (daily for 6 to 7 wk) increased GHBP activity (P less than .05). However, periodic short-term pST injections (daily, every 2nd d, or every 4th d for 2 wk) did not cause a significant change in GHBP levels (P greater than .40). Although fasting seems to reduce liver GH receptors, no difference was observed between fed animals and animals fasted for 5 d (P greater than .30). Between 0 and 6 mo of age, boar and gilt serum GHBP activity were not significantly different from each other but increased with age in both sexes (P less than .0001). There was no significant correlation between serum GHBP and BW at 6 mo of age in this study (P greater than .30). In pregnant sows, GHBP concentrations were highest at the beginning (d 72) of the third trimester (P less than .05). Growth hormone receptor activity reported by other researchers and GHBP activity in this study seem to vary similarly except during fasting, which may indicate alternate regulation of either the GHBP or the GH receptor.