-transparent.png){kind=logo}
Lymphocyte immunophenotyping by flow cytometry in normal adults. Comparison of fresh whole blood lysis technique, Ficoll-Paque separation and cryopreservation. 1992
M A Romeu, and
M Mestre, and
L González, and
A Valls, and
J Verdaguer, and
M Corominas, and
J Bas, and
E Massip, and
E Buendia
Servei d'Immunologia, Hospital Duran i Reynals, Barcelona, Catalunya, Spain.
In the present report we have assessed the extent to which Ficoll-Paque separation and cryopreservation of mononuclear cells alter the measurement of lymphocyte subsets by flow cytometry. Standard Ficoll-Paque separation increased the percentage of CD4+, CD19+ and CD4+CD45RA+ cells, as well as decreasing that of CD8+, and CD4+CD29+ cells, compared to the fresh whole blood lysis technique. Moreover, cryopreservation caused a depletion of CD4+ p80+ cells, but normal whole blood values were restored following a short incubation.
Related Publications
M A Romeu, and
M Mestre, and
L González, and
A Valls, and
J Verdaguer, and
M Corominas, and
J Bas, and
E Massip, and
E Buendia
April 1987,
Journal of immunological methods,
M A Romeu, and
M Mestre, and
L González, and
A Valls, and
J Verdaguer, and
M Corominas, and
J Bas, and
E Massip, and
E Buendia
May 2022,
Cells,
M A Romeu, and
M Mestre, and
L González, and
A Valls, and
J Verdaguer, and
M Corominas, and
J Bas, and
E Massip, and
E Buendia
January 1980,
Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B,
M A Romeu, and
M Mestre, and
L González, and
A Valls, and
J Verdaguer, and
M Corominas, and
J Bas, and
E Massip, and
E Buendia
January 2018,
Journal of immunological methods,
M A Romeu, and
M Mestre, and
L González, and
A Valls, and
J Verdaguer, and
M Corominas, and
J Bas, and
E Massip, and
E Buendia
April 2006,
Journal of immunological methods,
M A Romeu, and
M Mestre, and
L González, and
A Valls, and
J Verdaguer, and
M Corominas, and
J Bas, and
E Massip, and
E Buendia
April 2018,
International journal of laboratory hematology,
M A Romeu, and
M Mestre, and
L González, and
A Valls, and
J Verdaguer, and
M Corominas, and
J Bas, and
E Massip, and
E Buendia
January 1990,
Cytometry,
M A Romeu, and
M Mestre, and
L González, and
A Valls, and
J Verdaguer, and
M Corominas, and
J Bas, and
E Massip, and
E Buendia
January 1984,
International archives of allergy and applied immunology,
M A Romeu, and
M Mestre, and
L González, and
A Valls, and
J Verdaguer, and
M Corominas, and
J Bas, and
E Massip, and
E Buendia
June 2023,
Phenomics (Cham, Switzerland),
M A Romeu, and
M Mestre, and
L González, and
A Valls, and
J Verdaguer, and
M Corominas, and
J Bas, and
E Massip, and
E Buendia
April 2002,
Cytometry,
