Biomaterial Database

Micromonospora RNA polymerase activity changes during stationary phase. 1992

L S Lin, and D M Rothstein

Department of Microbial Genetics, Lederle Laboratories, Pearl River, NY 10965.

RNA polymerase was isolated from Micromonospora echinospora and from Streptomyces lividans. In vitro transcription of a DNA fragment containing multiple tandem promoters from Micromonospora followed the pattern of expression observed previously for in vivo studies. RNA polymerase was prepared from cultures of Micromonospora that were harvested during the growing phase and during the stationary phase. Promoters that were utilized in Micromonospora only during the stationary phase were utilized in vitro only when RNA polymerase was purified from a stationary-phase culture, and not when RNA polymerase was purified from growing cells.

UI	MeSH Term	Description	Entries
D008847	Micromonospora	A genus of gram-positive bacteria that forms a branched mycelium. It commonly occurs as a saprophytic form in soil and aquatic environments.	Micromonospora echinospora
D012321	DNA-Directed RNA Polymerases	Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).	DNA-Dependent RNA Polymerases,RNA Polymerases,Transcriptases,DNA-Directed RNA Polymerase,RNA Polymerase,Transcriptase,DNA Dependent RNA Polymerases,DNA Directed RNA Polymerase,DNA Directed RNA Polymerases,Polymerase, DNA-Directed RNA,Polymerase, RNA,Polymerases, DNA-Dependent RNA,Polymerases, DNA-Directed RNA,Polymerases, RNA,RNA Polymerase, DNA-Directed,RNA Polymerases, DNA-Dependent,RNA Polymerases, DNA-Directed
D013302	Streptomyces	A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.
D014158	Transcription, Genetic	The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.	Genetic Transcription