-transparent.png){kind=logo}
Monoclonal antibodies (MAbs) were produced against arabis mosaic nepovirus (AMV). A hybridoma screening procedure was applied which involved the testing of culture supernatants, before the hybridomas were cloned to single cell lines, for their reaction with eight nepoviruses [AMV, cherry leafroll virus (CLRV), grapevine fanleaf virus (GFLV), peach rosette mosaic virus, raspberry ringspot virus (RRSV), tobacco ringspot virus, tomato black ring virus (TBRV) and tomato ringspot virus]. In addition to AMV-specific MAbs, this screening technique has allowed the selection of two cross-reacting MAbs: one reacting with AMV and GFLV, and one reacting with AMV and RRSV. This is the first report of MAbs cross-reacting with these nepoviruses. In addition, five heterospecific MAbs (HS-MAbs) could be selected: two reacting with RRSV, two with CLRV and one with TBRV. The usefulness of the screening technique that was applied for the selection of cross-reacting MAbs and HS-MAbs, and the potential use of such antibodies are discussed.
E A Frison, and
R Stace-Smith
October 1957,
Blood,
E A Frison, and
R Stace-Smith
January 1991,
Archives of virology,
E A Frison, and
R Stace-Smith
January 1986,
Archives of virology,
E A Frison, and
R Stace-Smith
June 1990,
The Journal of general virology,
E A Frison, and
R Stace-Smith
January 1987,
Advances in experimental medicine and biology,
E A Frison, and
R Stace-Smith
January 1983,
Oncodevelopmental biology and medicine : the journal of the International Society for Oncodevelopmental Biology and Medicine,
E A Frison, and
R Stace-Smith
December 1984,
Cellular and molecular neurobiology,
E A Frison, and
R Stace-Smith
January 1993,
Plant molecular biology,
E A Frison, and
R Stace-Smith
October 1989,
Journal of clinical microbiology,
E A Frison, and
R Stace-Smith
April 2013,
Journal of structural biology,
