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The blue luminescence characteristic of the marine ostracod crustacean Vargula hilgendorfii is from a simple, but highly specific, enzyme-substrate reaction. Light is emitted by the oxidation of Vargula luciferin (substrate) by molecular oxygen, a reaction catalyzed by luciferase. Stable transformants of Chinese hamster ovary cells carrying the Vargula luciferase gene secreted luciferase from discrete sites on the cell surface, and this secretion could be monitored in real time by the bioluminescence produced by the secreted luciferase in the presence of Vargula luciferin by using an image-intensifying technique. Addition of anti-Vargula luciferase IgG to the luminescing cells almost completely extinguished the luminescence, confirming that Vargula luciferase caused the luminescence.
S Inouye, and
Y Ohmiya, and
Y Toya, and
F I Tsuji
January 2022,
Metabolic engineering,
S Inouye, and
Y Ohmiya, and
Y Toya, and
F I Tsuji
January 1987,
Methods in enzymology,
S Inouye, and
Y Ohmiya, and
Y Toya, and
F I Tsuji
July 2023,
Analytical and bioanalytical chemistry,
S Inouye, and
Y Ohmiya, and
Y Toya, and
F I Tsuji
January 1984,
Journal of interferon research,
S Inouye, and
Y Ohmiya, and
Y Toya, and
F I Tsuji
March 2006,
The Biochemical journal,
S Inouye, and
Y Ohmiya, and
Y Toya, and
F I Tsuji
June 2015,
BMC biotechnology,
S Inouye, and
Y Ohmiya, and
Y Toya, and
F I Tsuji
July 1998,
Artificial cells, blood substitutes, and immobilization biotechnology,
S Inouye, and
Y Ohmiya, and
Y Toya, and
F I Tsuji
January 1995,
Methods in molecular biology (Clifton, N.J.),
S Inouye, and
Y Ohmiya, and
Y Toya, and
F I Tsuji
July 2002,
Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
S Inouye, and
Y Ohmiya, and
Y Toya, and
F I Tsuji
April 1978,
Experimental cell research,
