Biomaterial Database

[Energization of membrane vesicles from the cells of glycolyzing bacterium Streptococcus faecalis]. 1976

G A Gorneva, and S N Skopinskaia, and V V Demin, and I D Riabova

Membrane fractions were isolated from Streptococcus faecalis cells of a glycolyzing microorganism, devoid of the respiratory chain, using the methods of osmotic shock of the protoplasts, ultrasonic treatment of the cells and ultrasonic treatment of the protoplasts. All fractions possessed the ATPase activity, the highest activity being observed in the fraction isolated by ultrasonication of the protoplasts. All preparations were estimated with respect to the presence of vesicles, formed by the "inside-out" and "inside-in" membranes, using ATPase as a marker of the membrane orientation. In the membrane fractions obtained by ultrasonication of the protoplasts, the "inside-out" vesicles were prevalent. ATP-dependent energization of the membranes, sensitive to the action of dicyclohexylcarbodiimide and tetrachlorotrifluoromethyl benzimidazole, was demonstrated by measuring the transport of the lipophylic anion of phenyldicarbaundecaborane and aniline naphthalene sulfonate fluorescence.

UI	MeSH Term	Description	Entries
D011523	Protoplasts	The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.	Protoplast
D001896	Boron Compounds	Inorganic or organic compounds that contain boron as an integral part of the molecule.	Borides,Compounds, Boron
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D004024	Dicyclohexylcarbodiimide	A carbodiimide that is used as a chemical intermediate and coupling agent in peptide synthesis. (From Hawley's Condensed Chemical Dictionary, 12th ed)	DCCD
D004734	Energy Metabolism	The chemical reactions involved in the production and utilization of various forms of energy in cells.	Bioenergetics,Energy Expenditure,Bioenergetic,Energy Expenditures,Energy Metabolisms,Expenditure, Energy,Expenditures, Energy,Metabolism, Energy,Metabolisms, Energy
D000251	Adenosine Triphosphatases	A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.	ATPases,Adenosinetriphosphatase,ATPase,ATPase, DNA-Dependent,Adenosine Triphosphatase,DNA-Dependent ATPase,DNA-Dependent Adenosinetriphosphatases,ATPase, DNA Dependent,Adenosinetriphosphatases, DNA-Dependent,DNA Dependent ATPase,DNA Dependent Adenosinetriphosphatases,Triphosphatase, Adenosine
D000255	Adenosine Triphosphate	An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.	ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D000817	Anilino Naphthalenesulfonates	A class of organic compounds which contain an anilino (phenylamino) group linked to a salt or ester of naphthalenesulfonic acid. They are frequently used as fluorescent dyes and sulfhydryl reagents.	Naphthalenesulfonates, Anilino
D001562	Benzimidazoles	Compounds with a BENZENE fused to IMIDAZOLES.
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence

Related Publications

G A Gorneva, and S N Skopinskaia, and V V Demin, and I D Riabova

A resonance Raman and electronic absorption probe of membrane energization. Quinaldine red in cells of Streptococcus faecalis.

October 1979, The Journal of biological chemistry,

G A Gorneva, and S N Skopinskaia, and V V Demin, and I D Riabova

ATP-linked calcium transport in cells and membrane vesicles of Streptococcus faecalis.

April 1978, The Journal of biological chemistry,

G A Gorneva, and S N Skopinskaia, and V V Demin, and I D Riabova

Aminoglycoside accumulation by membrane vesicles of Escherichia coli and Streptococcus faecalis.

May 1983, Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...,

G A Gorneva, and S N Skopinskaia, and V V Demin, and I D Riabova

Energization of solute transport in membrane vesicles from anaerobically grown bacteria.

January 1979, Methods in enzymology,

G A Gorneva, and S N Skopinskaia, and V V Demin, and I D Riabova

Protoplast membrane of Streptococcus faecalis.

January 1963, Journal of bacteriology,

G A Gorneva, and S N Skopinskaia, and V V Demin, and I D Riabova

THE PHOSPHOLIPIDS IN MEMBRANE GHOSTS FROM STREPTOCOCCUS FAECALIS PROTOPLASTS.

December 1964, Biochemistry,

G A Gorneva, and S N Skopinskaia, and V V Demin, and I D Riabova

Energization of amino acid transport in energy-depleted Ehrlich cells and plasma membrane vesicles.

June 1980, Biochimica et biophysica acta,