-transparent.png){kind=logo}
A procedure for the isolation of plasma-membrane-enriched fractions from bovine 'pars intermedia' and neurohypophysis is described. Various fractions are isolated by differential centrifugation and discontinuous sucrose density gradients. The plasma-membrane-enriched fractions have a density in sucrose of 1.14 and 1.16 and the yields are 1.8 mg and 1.5 mg per gram of tissue for the pars intermedia and neural lobe, respectively. The fractions are characterized by electron microscopy and enzymatic assays. The plasma membrane fractions are mainly vesicular in nature and are free of nuclei, mitochondria, and microsomes when examined by electron microscopy. 5'-Nucleotidase (EC 3.1.3.5) and Mg2+-(Na+ + K+)-ATPase (EC 3.6.1.3) activities are concentrated in the plasma-membrane-enriched fraction. Also, adenylate cyclase (EC 4.61.1) shows a 5 to 10-fold purification in the isolated membrane fraction. NaF (10mM) gives a two to three-fold stimulation of enzymatic activity in all fractions studied The yields of adenylate cyclase, 5'-nucleotidase, and Mg2+-(Na+ +K+)-ATPase are about 6% in the membrane fraction.
G Poirier, and
F Labrie, and
A Lemay, and
A Dupont, and
M Savary, and
G Pelletier
January 1982,
Ukrainskii biokhimicheskii zhurnal (1978),
G Poirier, and
F Labrie, and
A Lemay, and
A Dupont, and
M Savary, and
G Pelletier
June 1971,
The Journal of biological chemistry,
G Poirier, and
F Labrie, and
A Lemay, and
A Dupont, and
M Savary, and
G Pelletier
July 1983,
Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire,
G Poirier, and
F Labrie, and
A Lemay, and
A Dupont, and
M Savary, and
G Pelletier
August 1971,
Proceedings of the National Academy of Sciences of the United States of America,
G Poirier, and
F Labrie, and
A Lemay, and
A Dupont, and
M Savary, and
G Pelletier
July 1985,
Biochemistry,
G Poirier, and
F Labrie, and
A Lemay, and
A Dupont, and
M Savary, and
G Pelletier
November 1985,
Molecular and cellular endocrinology,
G Poirier, and
F Labrie, and
A Lemay, and
A Dupont, and
M Savary, and
G Pelletier
November 1981,
The Journal of biological chemistry,
G Poirier, and
F Labrie, and
A Lemay, and
A Dupont, and
M Savary, and
G Pelletier
December 1985,
The EMBO journal,
G Poirier, and
F Labrie, and
A Lemay, and
A Dupont, and
M Savary, and
G Pelletier
July 1974,
The Journal of cell biology,
G Poirier, and
F Labrie, and
A Lemay, and
A Dupont, and
M Savary, and
G Pelletier
July 1981,
The Biochemical journal,
