Biomaterial Database

Study on the prevention of surface-induced platelet activation by albumin coating. 1992

M Amiji, and H Park, and K Park

Purdue University, School of Pharmacy, West Lafayette, In 47907.

To understand how albumin on the surface inhibits surface-induced platelet activation, we adsorbed albumin on dimethyldichlorosilane-coated glass (DDS-glass) and modified the adsorbed albumin by three different methods. The adsorbed albumin was crosslinked with glutaraldehyde, dried and rehydrated, or digested with trypsin. Surface albumin concentration did not change by crosslinking; however, it decreased by about 15% by a simple dry-and-rehydration process. Trypsin digestion reduced the surface albumin concentration by 50%. Platelets were found to adhere and activate on albumin coated DDS-glass, if the adsorbed albumin was modified. The extent of platelet activation was quantified with two numeric parameters, the spread area and circularity. Fibrinogen adsorption to the dried or digested albumin layer resulted in enhancement of platelet activation, while adsorption of more albumin inhibited platelet activation. The results suggest that albumin can inhibit platelet activation as long as it covers the surface completely and remains flexible on the surface. This study indicates that steric repulsion is one of the mechanisms of surface passivation by albumin.

UI	MeSH Term	Description	Entries
D008855	Microscopy, Electron, Scanning	Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.	Scanning Electron Microscopy,Electron Scanning Microscopy,Electron Microscopies, Scanning,Electron Microscopy, Scanning,Electron Scanning Microscopies,Microscopies, Electron Scanning,Microscopies, Scanning Electron,Microscopy, Electron Scanning,Microscopy, Scanning Electron,Scanning Electron Microscopies,Scanning Microscopies, Electron,Scanning Microscopy, Electron
D010973	Platelet Adhesiveness	The process whereby PLATELETS adhere to something other than platelets, e.g., COLLAGEN; BASEMENT MEMBRANE; MICROFIBRILS; or other "foreign" surfaces.	Adhesiveness, Platelet,Adhesivenesses, Platelet,Platelet Adhesivenesses
D003890	Desiccation	Removal of moisture from a substance (chemical, food, tissue, etc.).	Dessication
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000327	Adsorption	The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.	Adsorptions
D012709	Serum Albumin	A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.	Plasma Albumin,Albumin, Serum
D013499	Surface Properties	Characteristics or attributes of the outer boundaries of objects, including molecules.	Properties, Surface,Property, Surface,Surface Property
D014357	Trypsin	A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.	Tripcellim,Trypure,beta-Trypsin,beta Trypsin
D014867	Water	A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Hydrogen Oxide
D015539	Platelet Activation	A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.	Activation, Platelet,Activations, Platelet,Platelet Activations

Related Publications

M Amiji, and H Park, and K Park

Effect of complement inhibition and heparin coating on artificial surface-induced leukocyte and platelet activation.

March 2004, The Annals of thoracic surgery,

M Amiji, and H Park, and K Park

Mechanistic Study on the Reduction of SWCNT-induced Cytotoxicity by Albumin Coating.

December 2014, Particle & particle systems characterization : measurement and description of particle properties and behavior in powders and other disperse systems,

M Amiji, and H Park, and K Park

Platelet-surface interaction: effect of albumin coating or heparin complexing on thrombogenic surfaces.

April 1974, Canadian journal of physiology and pharmacology,

M Amiji, and H Park, and K Park

Inhibition of surface-induced platelet activation by nitric oxide.

January 1995, ASAIO journal (American Society for Artificial Internal Organs : 1992),

M Amiji, and H Park, and K Park

Potentiation of liposome-induced complement activation by surface-bound albumin.

February 2002, Biochimica et biophysica acta,

M Amiji, and H Park, and K Park

Reduction of surface-induced platelet activation on phospholipid polymer.

September 1997, Journal of biomedical materials research,