BIOMAT Database Logo BIOMAT Database Logo

Modifications of adhesion properties and proteoglycan structure in rat embryo fibroblast cultures with increasing passages. 1992

P Pippia, and M Formato, and L Sciola, and G Tilloca, and A Bardoni, and G Pallavicini, and A Passi, and S Rindi, and R Salvini, and G De Luca
Institute of General Physiology and Biological Chemistry, University of Sassari, Italy.

Adhesion properties of rat embryo fibroblast cultures and proteoglycans (PGs) produced both in the growth medium and in the cell layer were investigated with increasing passages. Both cell-cell and cell-substrate adhesion increased with increasing subculture number. Cell adhesion properties were improved by cell treatment with chondroitinase ABC. The increase in subculture number was coupled with a constant increase of PG molecular size, which was particularly evident in cell layer extracts. The ratio HS-PGs/DS-PGs increased with increasing passages. PG modifications are likely to represent evidence of changes in extracellular matrix organization and could play a role in the increase of cell adhesion properties.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D011509 Proteoglycans Glycoproteins which have a very high polysaccharide content. Proteoglycan,Proteoglycan Type H
D002448 Cell Adhesion Adherence of cells to surfaces or to other cells. Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D002450 Cell Communication Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP. Cell Interaction,Cell-to-Cell Interaction,Cell Communications,Cell Interactions,Cell to Cell Interaction,Cell-to-Cell Interactions,Communication, Cell,Communications, Cell,Interaction, Cell,Interaction, Cell-to-Cell,Interactions, Cell,Interactions, Cell-to-Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004622 Embryo, Mammalian The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS. Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D005109 Extracellular Matrix A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere. Matrix, Extracellular,Extracellular Matrices,Matrices, Extracellular
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

P Pippia, and M Formato, and L Sciola, and G Tilloca, and A Bardoni, and G Pallavicini, and A Passi, and S Rindi, and R Salvini, and G De Luca
[Changes in the vaccinia virus through continuing passages in chick embryo fibroblast cultures].
December 1964, Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-hygienische Bakteriologie, Virusforschung und Parasitologie. Originale,
P Pippia, and M Formato, and L Sciola, and G Tilloca, and A Bardoni, and G Pallavicini, and A Passi, and S Rindi, and R Salvini, and G De Luca
The effect of charged synthetic polymers on proteoglycan synthesis and sequestration in chick embryo fibroblast cultures.
May 1979, Biochimica et biophysica acta,
P Pippia, and M Formato, and L Sciola, and G Tilloca, and A Bardoni, and G Pallavicini, and A Passi, and S Rindi, and R Salvini, and G De Luca
Proteoglycan alterations in skin fibroblast cultures from patients affected with pseudoxanthoma elasticum.
June 1996, Cell biochemistry and function,
P Pippia, and M Formato, and L Sciola, and G Tilloca, and A Bardoni, and G Pallavicini, and A Passi, and S Rindi, and R Salvini, and G De Luca
Cytopathic and pathogenic properties of dengue type 2 virus reproduced in quail embryo fibroblast cultures.
September 1980, Acta virologica,
P Pippia, and M Formato, and L Sciola, and G Tilloca, and A Bardoni, and G Pallavicini, and A Passi, and S Rindi, and R Salvini, and G De Luca
Eosinophil cationic protein alters proteoglycan metabolism in human lung fibroblast cultures.
December 1992, European journal of cell biology,
P Pippia, and M Formato, and L Sciola, and G Tilloca, and A Bardoni, and G Pallavicini, and A Passi, and S Rindi, and R Salvini, and G De Luca
Structure, modifications and ligand-binding properties of rat profilin 2a.
April 2009, Acta crystallographica. Section D, Biological crystallography,
P Pippia, and M Formato, and L Sciola, and G Tilloca, and A Bardoni, and G Pallavicini, and A Passi, and S Rindi, and R Salvini, and G De Luca
Human interferon: large scale production in embryo fibroblast cultures.
January 1978, Advances in experimental medicine and biology,
P Pippia, and M Formato, and L Sciola, and G Tilloca, and A Bardoni, and G Pallavicini, and A Passi, and S Rindi, and R Salvini, and G De Luca
Rat embryo fibroblast complementation assay with ras genes.
January 1995, Methods in enzymology,
P Pippia, and M Formato, and L Sciola, and G Tilloca, and A Bardoni, and G Pallavicini, and A Passi, and S Rindi, and R Salvini, and G De Luca
Formation of proteoglycan aggregates in rat chondrosarcoma chondrocyte cultures treated with tunicamycin.
October 1983, The Journal of biological chemistry,
P Pippia, and M Formato, and L Sciola, and G Tilloca, and A Bardoni, and G Pallavicini, and A Passi, and S Rindi, and R Salvini, and G De Luca
Proteoglycan synthesis in flat cell-free cultures of chick embryo retinal neurons and photoreceptors.
April 1988, Developmental biology,
Copied contents to your clipboard!