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1. The proteolytic processing sites of human lysosomal aspartic protease cathepsin D at which the intermediate single-chain form was converted into the mature two-chain form were determined. 2. The two chains were isolated by reversed-phase HPLC in order to investigate the cleavage sites of the enzyme. 3. Protein sequencing of the heavy chain, which was presumed to be derived from the C-terminal side in the single-chain enzyme, gave an N-terminal Leu 105. In addition, it revealed that there were also minor sequences, which commenced with Gly 106 and Gly 107. 4. A small C-terminal peptide was isolated from the light chain, which had been digested with two kinds of exogenous proteases. Sequence determination of this peptide, which was characterized as a nonapeptide by mass spectrometry, suggested that the C-terminus of the light chain was Ser 98. 5. These results indicate that a Ser 98-Ala 99 bond and an Ala 104-Leu 105 bond are cleaved to release 6 amino acid residues between the two chains.
T Kobayashi, and
K Honke, and
S Gasa, and
T Fujii, and
S Maguchi, and
T Miyazaki, and
A Makita
January 1995,
Neurochemistry international,
T Kobayashi, and
K Honke, and
S Gasa, and
T Fujii, and
S Maguchi, and
T Miyazaki, and
A Makita
March 2006,
Virology,
T Kobayashi, and
K Honke, and
S Gasa, and
T Fujii, and
S Maguchi, and
T Miyazaki, and
A Makita
June 1994,
European journal of cell biology,
T Kobayashi, and
K Honke, and
S Gasa, and
T Fujii, and
S Maguchi, and
T Miyazaki, and
A Makita
November 1988,
The Journal of biological chemistry,
T Kobayashi, and
K Honke, and
S Gasa, and
T Fujii, and
S Maguchi, and
T Miyazaki, and
A Makita
January 1985,
The American journal of physiology,
T Kobayashi, and
K Honke, and
S Gasa, and
T Fujii, and
S Maguchi, and
T Miyazaki, and
A Makita
August 1992,
The Journal of biological chemistry,
T Kobayashi, and
K Honke, and
S Gasa, and
T Fujii, and
S Maguchi, and
T Miyazaki, and
A Makita
December 1990,
Biochemical and biophysical research communications,
T Kobayashi, and
K Honke, and
S Gasa, and
T Fujii, and
S Maguchi, and
T Miyazaki, and
A Makita
November 1997,
Journal of immunology (Baltimore, Md. : 1950),
T Kobayashi, and
K Honke, and
S Gasa, and
T Fujii, and
S Maguchi, and
T Miyazaki, and
A Makita
October 1983,
Biochemistry,
T Kobayashi, and
K Honke, and
S Gasa, and
T Fujii, and
S Maguchi, and
T Miyazaki, and
A Makita
March 1985,
The Journal of biological chemistry,
