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A flow-cytofluorometric method, based on the differential stability of deoxyribonucleic acid versus ribonucleic acid with the metachromatic dye, acridine orange, simultaneously measures the following parameters of stimulation in mixed lymphocyte cultures: (a) number of nonstimulated cells; (b) total number of stimulated lymphocytes; (c) number of stimulated lymphocytes in G1, S and G2 + M phases of the cell cycle; (d) number of macrophages; (e) number of dead cells. The progress of lymphocyte stimulation may also be measured by a parameter representing ribonucleic acid accumulation per cell. The method is rapid, avoids cell rinsing, fixation and centrifugation and is applicable to microcultures. Multiparameter analysis of cell stimulation which provides simultaneous measurements of lymphocyte proliferation and accumulation of ribonucleic acid per cell may prove to be a more sensitive assay of histocompatibility than tests based only on cell proliferation (tritiated thymidine incorporation).
F Traganos, and
A J Gorski, and
Z Darzynkiewicz, and
T Sharpless, and
M R Melamed
August 1976,
Proceedings of the National Academy of Sciences of the United States of America,
F Traganos, and
A J Gorski, and
Z Darzynkiewicz, and
T Sharpless, and
M R Melamed
October 2000,
Cytometry,
F Traganos, and
A J Gorski, and
Z Darzynkiewicz, and
T Sharpless, and
M R Melamed
July 1976,
Horumon to rinsho. Clinical endocrinology,
F Traganos, and
A J Gorski, and
Z Darzynkiewicz, and
T Sharpless, and
M R Melamed
November 1981,
British journal of cancer,
F Traganos, and
A J Gorski, and
Z Darzynkiewicz, and
T Sharpless, and
M R Melamed
August 1970,
Clinical and experimental immunology,
F Traganos, and
A J Gorski, and
Z Darzynkiewicz, and
T Sharpless, and
M R Melamed
May 1982,
Tissue antigens,
F Traganos, and
A J Gorski, and
Z Darzynkiewicz, and
T Sharpless, and
M R Melamed
September 1972,
The Tohoku journal of experimental medicine,
F Traganos, and
A J Gorski, and
Z Darzynkiewicz, and
T Sharpless, and
M R Melamed
January 1984,
Cellular immunology,
F Traganos, and
A J Gorski, and
Z Darzynkiewicz, and
T Sharpless, and
M R Melamed
April 1986,
Transplantation proceedings,
F Traganos, and
A J Gorski, and
Z Darzynkiewicz, and
T Sharpless, and
M R Melamed
August 2018,
SLAS discovery : advancing life sciences R & D,
