[Quantitative isolation of acid mucopolysaccharides from urine in progressive scleroderma (author's transl)]. 1977

H W Kreysel, and H Kraus, and H W Stuhlsatz, and E Greiling, and H P Nissen, and J Kimmig

After filtration the urine was concentrated to about 1/10 of the original volume. The urine was centrifuged for 15 min at 5000 rev/min. The supernatant was chromatographed on Bio-Gel P-2-with a 10% aqueous solution of ethanol. Then the glycosaminoglycan fraction was separated into seven further fractions by chromatography on Dowex 1 X 2 and elution with increasing concentration of sodium chlorid. The desalted fractions were analysed. From the analytical data presented no definite correlation between excretion of glycosaminoglycans in urine and progressive scleroderma can be observed. These results were discussed with further data presented by Hexosamine determination.

UI MeSH Term Description Entries
D011509 Proteoglycans Glycoproteins which have a very high polysaccharide content. Proteoglycan,Proteoglycan Type H
D006025 Glycosaminoglycans Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine (see ACETYLGLUCOSAMINE) or N-acetylgalactosamine (see ACETYLGALACTOSAMINE). Glycosaminoglycan,Mucopolysaccharides
D006595 Hexosamines AMINO SUGARS created by adding an amine group to a hexose sugar. Hexosamine
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012595 Scleroderma, Systemic A chronic multi-system disorder of CONNECTIVE TISSUE. It is characterized by SCLEROSIS in the SKIN, the LUNGS, the HEART, the GASTROINTESTINAL TRACT, the KIDNEYS, and the MUSCULOSKELETAL SYSTEM. Other important features include diseased small BLOOD VESSELS and AUTOANTIBODIES. The disorder is named for its most prominent feature (hard skin), and classified into subsets by the extent of skin thickening: LIMITED SCLERODERMA and DIFFUSE SCLERODERMA. Sclerosis, Systemic,Systemic Scleroderma,Systemic Sclerosis
D014574 Uronic Acids Acids derived from monosaccharides by the oxidation of the terminal (-CH2OH) group farthest removed from the carbonyl group to a (-COOH) group. (From Stedmans, 26th ed) Uronic Acid,Acid, Uronic,Acids, Uronic

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