-transparent.png){kind=logo}
A buoyant density gradient procedure for mammalian cell separation is described. Gradients of Ficoll and Hypaque in balanced salt solution were used in a zonal rotor to separate distinct leukocyte populations from human peripheral blood and bone marrow. This method possesses the advantages of uniform osmolarity, density determination by refractometry, ease of preparation of gradient solutions, large sample capacity, and low viscosity with consequent ease of sterilization. Granulocyte-monocyte colony-forming units (CFU-C) from blood and bone marrow banded as a homogeneous peak with a density of 1.056 g cm-3 on steep gradients. Recovery of CFU-C was 36% with a 7- to 9-fold enrichment on shallow gradients. Both fractionated and unfractionated peripheral blood cells were stimulated by phytohemagglutinin (PHA), Concanavalin A (Con A), and allogeneic lymphocytes. In contrast, cells from unfractionated bone marrow and cells from the light density fraction of marrow were inhibited by Con A and responded variably to PHA.
J R Wells, and
G Opelz, and
M J Cline
May 1975,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
J R Wells, and
G Opelz, and
M J Cline
April 1981,
Cellular immunology,
J R Wells, and
G Opelz, and
M J Cline
March 1964,
Annals of the New York Academy of Sciences,
J R Wells, and
G Opelz, and
M J Cline
June 1979,
Cryobiology,
J R Wells, and
G Opelz, and
M J Cline
September 1973,
Experimental cell research,
J R Wells, and
G Opelz, and
M J Cline
July 1976,
Blood,
J R Wells, and
G Opelz, and
M J Cline
December 1971,
Journal of cellular physiology,
J R Wells, and
G Opelz, and
M J Cline
January 1991,
Laboratornoe delo,
J R Wells, and
G Opelz, and
M J Cline
February 2007,
Experimental hematology,
J R Wells, and
G Opelz, and
M J Cline
February 1969,
Biochemical and biophysical research communications,
