BIOMAT Database Logo BIOMAT Database Logo

Effect of a phorbol ester on immunoreactive endothelin-1 release from cultured porcine aortic endothelial cells. 1992

Y Hattori, and K Kasai, and N Banba, and S Hattori, and T Nakamura, and S Shimoda
Department of Endocrinology, Internal Medicine, Dokkyo University School of Medicine, Tochigi, Japan.

This study was designed to examine how protein kinase C (PKC) regulates the release of endothelin-1 (ET-1) from cultured porcine aortic endothelial cells. We measured the release of immunoreactive (IR)-ET-1 from cells cultured for up to 72 h in the presence or absence of a phorbol ester TPA. The release of IR-ET-1 from control cells (no TPA) increased according to time for up to 72 h. In the presence of TPA, the release of IR-ET-1 from the cells was higher than the control level for up to 8 h, but was lower thereafter and reached a plateau after 48 h. TPA dose-dependently stimulated IR-ET-1 release during incubation for 4 h, but suppressed it after incubation for 72 h. Stimulation of PKC by diacylglycerol mimicked the early (4 h) action of TPA. On the other hand, pretreatment of cells with TPA to downregulate PKC significantly suppressed basal and thrombin- or FCS-stimulated IR-ET-1 release. These findings suggest that the activation of PKC is related to the stimulation of ET-1 release and that down-regulation of PKC leads to the suppression of ET-1 release from cultured endothelial cells.

UI MeSH Term Description Entries
D011493 Protein Kinase C An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters. Calcium Phospholipid-Dependent Protein Kinase,Calcium-Activated Phospholipid-Dependent Kinase,PKC Serine-Threonine Kinase,Phospholipid-Sensitive Calcium-Dependent Protein Kinase,Protein Kinase M,Calcium Activated Phospholipid Dependent Kinase,Calcium Phospholipid Dependent Protein Kinase,PKC Serine Threonine Kinase,Phospholipid Sensitive Calcium Dependent Protein Kinase,Phospholipid-Dependent Kinase, Calcium-Activated,Serine-Threonine Kinase, PKC
D011863 Radioimmunoassay Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation. Radioimmunoassays
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004075 Diglycerides Glycerides composed of two fatty acids esterified to the trihydric alcohol GLYCEROL. There are two possible forms that exist: 1,2-diacylglycerols and 1,3-diacylglycerols. Diacylglycerol,Diacylglycerols
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001011 Aorta The main trunk of the systemic arteries. Aortas
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D013755 Tetradecanoylphorbol Acetate A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA. Phorbol Myristate Acetate,12-Myristoyl-13-acetylphorbol,12-O-Tetradecanoyl Phorbol 13-Acetate,Tetradecanoylphorbol Acetate, 4a alpha-Isomer,12 Myristoyl 13 acetylphorbol,12 O Tetradecanoyl Phorbol 13 Acetate,13-Acetate, 12-O-Tetradecanoyl Phorbol,Acetate, Phorbol Myristate,Acetate, Tetradecanoylphorbol,Myristate Acetate, Phorbol,Phorbol 13-Acetate, 12-O-Tetradecanoyl,Tetradecanoylphorbol Acetate, 4a alpha Isomer
D016232 Endothelins 21-Amino-acid peptides produced by vascular endothelial cells and functioning as potent vasoconstrictors. The endothelin family consists of three members, ENDOTHELIN-1; ENDOTHELIN-2; and ENDOTHELIN-3. All three peptides contain 21 amino acids, but vary in amino acid composition. The three peptides produce vasoconstrictor and pressor responses in various parts of the body. However, the quantitative profiles of the pharmacological activities are considerably different among the three isopeptides. Endothelium-Derived Vasoconstrictor Factors,Endothelin,Vasoconstrictor Factors, Endothelium-Derived

Related Publications

Y Hattori, and K Kasai, and N Banba, and S Hattori, and T Nakamura, and S Shimoda
Thrombin enhances the release of endothelin from cultured porcine aortic endothelial cells.
June 1989, European journal of pharmacology,
Y Hattori, and K Kasai, and N Banba, and S Hattori, and T Nakamura, and S Shimoda
Release of immunoreactive endothelin from porcine aortic strips.
June 1990, Hypertension (Dallas, Tex. : 1979),
Y Hattori, and K Kasai, and N Banba, and S Hattori, and T Nakamura, and S Shimoda
Granulocyte-colony stimulating factor stimulates immunoreactive endothelin-1 release from cultured bovine endothelial cells.
November 1992, European journal of pharmacology,
Y Hattori, and K Kasai, and N Banba, and S Hattori, and T Nakamura, and S Shimoda
Ethanol stimulates immunoreactive endothelin-1 and -2 release from cultured human umbilical vein endothelial cells.
April 1992, Alcoholism, clinical and experimental research,
Y Hattori, and K Kasai, and N Banba, and S Hattori, and T Nakamura, and S Shimoda
Differential effect of cyclic GMP on the release of endothelin-1 from cultured endothelial cells and intact porcine aorta.
January 1991, Journal of cardiovascular pharmacology,
Y Hattori, and K Kasai, and N Banba, and S Hattori, and T Nakamura, and S Shimoda
Phorbol ester stimulates acetylcholine synthesis in cultured endothelial cells isolated from porcine cerebral microvessels.
August 1994, Brain research,
Y Hattori, and K Kasai, and N Banba, and S Hattori, and T Nakamura, and S Shimoda
Pressure enhances endothelin-1 release from cultured human endothelial cells.
March 1995, Hypertension (Dallas, Tex. : 1979),
Y Hattori, and K Kasai, and N Banba, and S Hattori, and T Nakamura, and S Shimoda
Release mechanism of endothelin-1 and big endothelin-1 after stimulation with thrombin in cultured porcine endothelial cells.
January 1992, Journal of vascular research,
Y Hattori, and K Kasai, and N Banba, and S Hattori, and T Nakamura, and S Shimoda
Propofol stimulates nitric oxide release from cultured porcine aortic endothelial cells.
May 1993, British journal of pharmacology,
Y Hattori, and K Kasai, and N Banba, and S Hattori, and T Nakamura, and S Shimoda
Endothelin-1 induces prostacyclin release from bovine aortic endothelial cells.
May 1991, Biochemical and biophysical research communications,
Copied contents to your clipboard!