Biomaterial Database

Regulation of ATP-citrate lyase at transcriptional and post-transcriptional levels in rat liver. 1992

K S Kim, and S W Park, and Y S Kim

Department of Biochemistry, Yonsei University College of Medicine, Seoul, Korea.

The amounts of ATP-citrate lyase in liver cytosol began to increase at 12 hours after refeeding a high-carbohydrate diet and further increased until 48 hours. The amounts of the ATP-citrate lyase mRNA began to increase at 6 hours and reached to a maximum level at 12 hours, followed by decrease to a very low level until 48 hours. The elevated amount of the ATP-citrate lyase mRNA reflected on the increase of ATP-citrate lyase content in the first 24 hours, but these two parameters were not paralleled thereafter. The transcriptional activity of ATP-citrate lyase gene in nuclei of rat liver began to increase at 4 hours and further increased to reach a maximum level of 24 fold at 12 hours, maintaining a high level of 17 fold until 48 hours. The elevation of transcriptional activity of ATP-citrate lyase gene preceded the increase of ATP-citrate lyase mRNA content in the liver cytosol by 2 hours, and its increasing pattern was similar to changes of mRNA content until 12 hours. However, while the transcriptional activity remained at a high level until 48 hours, the ATP-citrate lyase mRNA concentration in the cytosol decreased after 12 hours.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008297	Male		Males
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009838	Oligodeoxyribonucleotides	A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.	Oligodeoxynucleotide,Oligodeoxyribonucleotide,Oligodeoxynucleotides
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D003600	Cytosol	Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.	Cytosols
D004040	Dietary Carbohydrates	Carbohydrates present in food comprising digestible sugars and starches and indigestible cellulose and other dietary fibers. The former are the major source of energy. The sugars are in beet and cane sugar, fruits, honey, sweet corn, corn syrup, milk and milk products, etc.; the starches are in cereal grains, legumes (FABACEAE), tubers, etc. (From Claudio & Lagua, Nutrition and Diet Therapy Dictionary, 3d ed, p32, p277)	Carbohydrates, Dietary,Carbohydrate, Dietary,Dietary Carbohydrate
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001275	ATP Citrate (pro-S)-Lyase	An enzyme that, in the presence of ATP and COENZYME A, catalyzes the cleavage of citrate to yield acetyl CoA, oxaloacetate, ADP, and ORTHOPHOSPHATE. This reaction represents an important step in fatty acid biosynthesis. This enzyme was formerly listed as EC 4.1.3.8.	ATP Citrate Lyase,ATP Citrate Synthase,Citrate Cleavage Enzyme,ATP Citrate (pro-3S)-Lyase,ATP-Dependent Citrate Lyase,ATP Dependent Citrate Lyase,Citrate Lyase, ATP,Citrate Lyase, ATP-Dependent,Citrate Synthase, ATP,Cleavage Enzyme, Citrate,Lyase, ATP Citrate,Lyase, ATP-Dependent Citrate,Synthase, ATP Citrate
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA