BIOMAT Database Logo BIOMAT Database Logo

Crystallization and X-ray analysis of NH3-dependent NAD+ synthetase from Helicobacter pylori. 2003

Gil Bu Kang, and Yun Sik Kim, and Young Jun Im, and Seong-Hwan Rho, and Soo Hyun Eom
Department of Life Science, Kwangju Institute of Science and Technology (K-JIST), Gwangju 500-712, Korea.

The ubiquitous NAD(+) synthetase catalyzes the key step in the biosynthesis of nicotinamide adenine dinucleotide. NH3-dependent NAD(+) synthetase from Helicobacter pylori was purified to homogeneity and crystallized using PEG 1500 as a precipitant. The crystal diffracted up to a resolution of 2.3+ and was found to belong to space group C2 with unit cell dimensions of a = 93.8, b = 48.3, c = 64.2 A and alpha = gamma = 90, beta = 110.0 degrees.

UI MeSH Term Description Entries
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D003627 Data Interpretation, Statistical Application of statistical procedures to analyze specific observed or assumed facts from a particular study. Data Analysis, Statistical,Data Interpretations, Statistical,Interpretation, Statistical Data,Statistical Data Analysis,Statistical Data Interpretation,Analyses, Statistical Data,Analysis, Statistical Data,Data Analyses, Statistical,Interpretations, Statistical Data,Statistical Data Analyses,Statistical Data Interpretations
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D015971 Gene Expression Regulation, Enzymologic Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis. Enzymologic Gene Expression Regulation,Regulation of Gene Expression, Enzymologic,Regulation, Gene Expression, Enzymologic
D016480 Helicobacter pylori A spiral bacterium active as a human gastric pathogen. It is a gram-negative, urease-positive, curved or slightly spiral organism initially isolated in 1982 from patients with lesions of gastritis or peptic ulcers in Western Australia. Helicobacter pylori was originally classified in the genus CAMPYLOBACTER, but RNA sequencing, cellular fatty acid profiles, growth patterns, and other taxonomic characteristics indicate that the micro-organism should be included in the genus HELICOBACTER. It has been officially transferred to Helicobacter gen. nov. (see Int J Syst Bacteriol 1989 Oct;39(4):297-405). Campylobacter pylori,Campylobacter pylori subsp. pylori,Campylobacter pyloridis,Helicobacter nemestrinae
D018360 Crystallography, X-Ray The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) X-Ray Crystallography,Crystallography, X Ray,Crystallography, Xray,X Ray Crystallography,Xray Crystallography,Crystallographies, X Ray,X Ray Crystallographies
D019732 Amide Synthases Enzymes that catalyze the joining of either ammonia or an amide with another molecule, in which the linkage is in the form of a carbon-nitrogen bond. EC 6.3.1. Acid-Amide Ligases,Acid-Ammonia Ligases,Acid Amide Ligases,Acid Ammonia Ligases,Ligases, Acid-Amide,Ligases, Acid-Ammonia,Synthases, Amide

Related Publications

Gil Bu Kang, and Yun Sik Kim, and Young Jun Im, and Seong-Hwan Rho, and Soo Hyun Eom
Expression, purification, crystallization and preliminary X-ray analysis of an NAD-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori.
August 2008, Acta crystallographica. Section F, Structural biology and crystallization communications,
Gil Bu Kang, and Yun Sik Kim, and Young Jun Im, and Seong-Hwan Rho, and Soo Hyun Eom
Crystallization and preliminary X-ray diffraction analysis of spermidine synthase from Helicobacter pylori.
November 2004, Acta crystallographica. Section D, Biological crystallography,
Gil Bu Kang, and Yun Sik Kim, and Young Jun Im, and Seong-Hwan Rho, and Soo Hyun Eom
Crystallization and preliminary X-ray crystallographic analysis of type II dehydroquinase from Helicobacter pylori.
February 2001, Acta crystallographica. Section D, Biological crystallography,
Gil Bu Kang, and Yun Sik Kim, and Young Jun Im, and Seong-Hwan Rho, and Soo Hyun Eom
Crystallization and preliminary X-ray crystallographic analysis of aspartate 1-decarboxylase from Helicobacter pylori.
May 2002, Acta crystallographica. Section D, Biological crystallography,
Gil Bu Kang, and Yun Sik Kim, and Young Jun Im, and Seong-Hwan Rho, and Soo Hyun Eom
Purification, crystallization and preliminary X-ray studies of ClpX from Helicobacter pylori.
September 2003, Acta crystallographica. Section D, Biological crystallography,
Gil Bu Kang, and Yun Sik Kim, and Young Jun Im, and Seong-Hwan Rho, and Soo Hyun Eom
Crystallization and X-ray data collection of HP0902 from Helicobacter pylori 26695.
December 2011, Acta crystallographica. Section F, Structural biology and crystallization communications,
Gil Bu Kang, and Yun Sik Kim, and Young Jun Im, and Seong-Hwan Rho, and Soo Hyun Eom
Expression, purification, crystallization and preliminary X-ray analysis of an NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori.
August 2008, Acta crystallographica. Section F, Structural biology and crystallization communications,
Gil Bu Kang, and Yun Sik Kim, and Young Jun Im, and Seong-Hwan Rho, and Soo Hyun Eom
Crystallization and preliminary X-ray crystallographic analysis of UDP-N-acetylglucosamine acyltransferase from Helicobacter pylori.
May 2002, Acta crystallographica. Section D, Biological crystallography,
Gil Bu Kang, and Yun Sik Kim, and Young Jun Im, and Seong-Hwan Rho, and Soo Hyun Eom
Crystallization and preliminary x-ray crystallographic analysis of NAD+-dependent DNA ligase from Thermus filiformis.
March 2000, Acta crystallographica. Section D, Biological crystallography,
Gil Bu Kang, and Yun Sik Kim, and Young Jun Im, and Seong-Hwan Rho, and Soo Hyun Eom
Crystallization and preliminary X-ray crystallographic studies of phosphopantetheine adenylyltransferase from Helicobacter pylori.
March 2003, Acta crystallographica. Section D, Biological crystallography,
Copied contents to your clipboard!