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Hepatitis B surface antigen immunopurification using a plant-derived specific antibody produced in large scale. 2003
Rodolfo Valdés, and
Biunayki Reyes, and
Tatiana Alvarez, and
Janet García, and
José A Montero, and
Alejandro Figueroa, and
Leonardo Gómez, and
Sigifredo Padilla, and
Déborah Geada, and
Ma Carmen Abrahantes, and
Lamay Dorta, and
Damián Fernández, and
Otto Mendoza, and
Nadia Ramirez, and
Marylin Rodriguez, and
Merardo Pujol, and
Carlos Borroto, and
José Brito
Monoclonal Antibody Department, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurification of the recombinant HBsAg for vaccine purposes. This plant-derived antibody was obtained from different batches of 100-200kg of tobacco leaves and coupled to Sepharose CL-4B with high efficiency. The plant-derived antibody immunoaffinity matrix purification behavior (elution capacity, antigen purity, purification cycles, and ligand leakage) was comparable to that of its mouse-derived monoclonal antibody homolog. This result supports the feasibility of using this plant-derived antibody for the immunopurification of the Hepatitis B surface antigen for human use, opening a new possibility to overcome the constrain of monoclonal antibody production in mice.
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