Immature tumor angiogenesis in high-grade and high-stage renal cell carcinoma. 2003

Toshiaki Kinouchi, and Masayuki Mano, and Ikuyo Matsuoka, and Sae Kodama, and Tomomi Aoki, and Mina Okamoto, and Hisako Yamamura, and Michiyuki Usami, and Katsuhito Takahashi
Department of Urology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari-ku, Osaka, Japan.

OBJECTIVE To investigate the correlation between pathologic findings and maturation of the tumor neovasculature of renal cell carcinoma by immunohistochemical studies. METHODS Formalin-fixed and paraffin-embedded specimens from 25 randomly selected patients with renal cell carcinoma were stained with mouse monoclonal antibodies, anti-human CD31, anti-alpha smooth muscle actin (alphaSMA), and anti-human calponin by the indirect immunoperoxidase method. The microvessels were counted in six areas with the higher number of microvessels in each patient at 200x magnification (0.255 mm2 per area). RESULTS The number of CD31-positive microvessels in grade 3 tumors was significantly lower than those in grade 1 or 2 tumors (P = 0.003222 and P = 0.043217, respectively). The CD31-positive microvessel counts of those of higher stage, tumor size greater than 4.5 cm, or non-clear cell type were significantly lower than tumors of lower stage, size less than 4.6 cm, or clear cell type. In the grade 3 tumors, the expression ratio of the number of alphaSMA-positive microvessels to the number of CD31-positive microvessels was significantly decreased compared with grade 1 or 2 tumors (P = 0.000011 and P = 0.000000, respectively). The expression of calponin in the tumor neovasculature was not observed. The expression ratios of the number of alphaSMA-positive microvessels to the number of CD31-positive microvessels in higher stages, larger tumor sizes, or non-clear cell types were significantly decreased. CONCLUSIONS The tumor neovasculature of high-grade and high-stage tumors was immature. These results imply that high-grade tumors of renal cell carcinomas may be susceptible to antiangiogenesis therapy inducing apoptosis of immature tumor vessels.

UI MeSH Term Description Entries
D007124 Immunoenzyme Techniques Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens. Antibody Enzyme Technique, Unlabeled,Enzyme Immunoassay,Enzyme-Labeled Antibody Technique,Immunoassay, Enzyme,Immunoperoxidase Techniques,Peroxidase-Antiperoxidase Complex Technique,Peroxidase-Labeled Antibody Technique,Antibody Enzyme Technic, Unlabeled,Enzyme-Labeled Antibody Technic,Immunoenzyme Technics,Immunoperoxidase Technics,Peroxidase-Antiperoxidase Complex Technic,Peroxidase-Labeled Antibody Technic,Antibody Technic, Enzyme-Labeled,Antibody Technic, Peroxidase-Labeled,Antibody Technics, Enzyme-Labeled,Antibody Technics, Peroxidase-Labeled,Antibody Technique, Enzyme-Labeled,Antibody Technique, Peroxidase-Labeled,Antibody Techniques, Enzyme-Labeled,Antibody Techniques, Peroxidase-Labeled,Enzyme Immunoassays,Enzyme Labeled Antibody Technic,Enzyme Labeled Antibody Technique,Enzyme-Labeled Antibody Technics,Enzyme-Labeled Antibody Techniques,Immunoassays, Enzyme,Immunoenzyme Technic,Immunoenzyme Technique,Immunoperoxidase Technic,Immunoperoxidase Technique,Peroxidase Antiperoxidase Complex Technic,Peroxidase Antiperoxidase Complex Technique,Peroxidase Labeled Antibody Technic,Peroxidase Labeled Antibody Technique,Peroxidase-Antiperoxidase Complex Technics,Peroxidase-Antiperoxidase Complex Techniques,Peroxidase-Labeled Antibody Technics,Peroxidase-Labeled Antibody Techniques,Technic, Enzyme-Labeled Antibody,Technic, Immunoenzyme,Technic, Immunoperoxidase,Technic, Peroxidase-Antiperoxidase Complex,Technic, Peroxidase-Labeled Antibody,Technics, Enzyme-Labeled Antibody,Technics, Immunoenzyme,Technics, Immunoperoxidase,Technics, Peroxidase-Antiperoxidase Complex,Technics, Peroxidase-Labeled Antibody,Technique, Enzyme-Labeled Antibody,Technique, Immunoenzyme,Technique, Immunoperoxidase,Technique, Peroxidase-Antiperoxidase Complex,Technique, Peroxidase-Labeled Antibody,Techniques, Enzyme-Labeled Antibody,Techniques, Immunoenzyme,Techniques, Immunoperoxidase,Techniques, Peroxidase-Antiperoxidase Complex,Techniques, Peroxidase-Labeled Antibody
D007680 Kidney Neoplasms Tumors or cancers of the KIDNEY. Cancer of Kidney,Kidney Cancer,Renal Cancer,Cancer of the Kidney,Neoplasms, Kidney,Renal Neoplasms,Cancer, Kidney,Cancer, Renal,Cancers, Kidney,Cancers, Renal,Kidney Cancers,Kidney Neoplasm,Neoplasm, Kidney,Neoplasm, Renal,Neoplasms, Renal,Renal Cancers,Renal Neoplasm
D008297 Male Males
D008840 Microfilament Proteins Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell. Actin Binding Protein,Actin-Binding Protein,Actin-Binding Proteins,Microfilament Protein,Actin Binding Proteins,Binding Protein, Actin,Protein, Actin Binding,Protein, Actin-Binding,Protein, Microfilament,Proteins, Actin-Binding,Proteins, Microfilament
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009363 Neoplasm Proteins Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm. Proteins, Neoplasm
D009367 Neoplasm Staging Methods which attempt to express in replicable terms the extent of the neoplasm in the patient. Cancer Staging,Staging, Neoplasm,Tumor Staging,TNM Classification,TNM Staging,TNM Staging System,Classification, TNM,Classifications, TNM,Staging System, TNM,Staging Systems, TNM,Staging, Cancer,Staging, TNM,Staging, Tumor,System, TNM Staging,Systems, TNM Staging,TNM Classifications,TNM Staging Systems
D009389 Neovascularization, Pathologic A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions. Angiogenesis, Pathologic,Angiogenesis, Pathological,Neovascularization, Pathological,Pathologic Angiogenesis,Pathologic Neovascularization,Pathological Angiogenesis,Pathological Neovascularization
D002135 Calcium-Binding Proteins Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS. Calcium Binding Protein,Calcium-Binding Protein,Calcium Binding Proteins,Binding Protein, Calcium,Binding Proteins, Calcium,Protein, Calcium Binding,Protein, Calcium-Binding
D002196 Capillaries The minute vessels that connect arterioles and venules. Capillary Beds,Sinusoidal Beds,Sinusoids,Bed, Sinusoidal,Beds, Sinusoidal,Capillary,Capillary Bed,Sinusoid,Sinusoidal Bed

Related Publications

Toshiaki Kinouchi, and Masayuki Mano, and Ikuyo Matsuoka, and Sae Kodama, and Tomomi Aoki, and Mina Okamoto, and Hisako Yamamura, and Michiyuki Usami, and Katsuhito Takahashi
January 2005, Indian journal of cancer,
Toshiaki Kinouchi, and Masayuki Mano, and Ikuyo Matsuoka, and Sae Kodama, and Tomomi Aoki, and Mina Okamoto, and Hisako Yamamura, and Michiyuki Usami, and Katsuhito Takahashi
March 1981, Nihon Hinyokika Gakkai zasshi. The japanese journal of urology,
Toshiaki Kinouchi, and Masayuki Mano, and Ikuyo Matsuoka, and Sae Kodama, and Tomomi Aoki, and Mina Okamoto, and Hisako Yamamura, and Michiyuki Usami, and Katsuhito Takahashi
April 2011, The American journal of surgical pathology,
Toshiaki Kinouchi, and Masayuki Mano, and Ikuyo Matsuoka, and Sae Kodama, and Tomomi Aoki, and Mina Okamoto, and Hisako Yamamura, and Michiyuki Usami, and Katsuhito Takahashi
April 2022, European radiology,
Toshiaki Kinouchi, and Masayuki Mano, and Ikuyo Matsuoka, and Sae Kodama, and Tomomi Aoki, and Mina Okamoto, and Hisako Yamamura, and Michiyuki Usami, and Katsuhito Takahashi
October 2022, Scientific reports,
Toshiaki Kinouchi, and Masayuki Mano, and Ikuyo Matsuoka, and Sae Kodama, and Tomomi Aoki, and Mina Okamoto, and Hisako Yamamura, and Michiyuki Usami, and Katsuhito Takahashi
January 2016, Asian Pacific journal of cancer prevention : APJCP,
Toshiaki Kinouchi, and Masayuki Mano, and Ikuyo Matsuoka, and Sae Kodama, and Tomomi Aoki, and Mina Okamoto, and Hisako Yamamura, and Michiyuki Usami, and Katsuhito Takahashi
August 1998, Zhonghua yi xue za zhi = Chinese medical journal; Free China ed,
Toshiaki Kinouchi, and Masayuki Mano, and Ikuyo Matsuoka, and Sae Kodama, and Tomomi Aoki, and Mina Okamoto, and Hisako Yamamura, and Michiyuki Usami, and Katsuhito Takahashi
December 2021, Zhonghua yi xue za zhi,
Toshiaki Kinouchi, and Masayuki Mano, and Ikuyo Matsuoka, and Sae Kodama, and Tomomi Aoki, and Mina Okamoto, and Hisako Yamamura, and Michiyuki Usami, and Katsuhito Takahashi
July 1995, The American journal of pathology,
Toshiaki Kinouchi, and Masayuki Mano, and Ikuyo Matsuoka, and Sae Kodama, and Tomomi Aoki, and Mina Okamoto, and Hisako Yamamura, and Michiyuki Usami, and Katsuhito Takahashi
January 2022, Case reports in oncology,
Copied contents to your clipboard!