BIOMAT Database Logo BIOMAT Database Logo

Transcriptional activation of c-fos and c-jun protooncogenes by serum growth factors in osteoblast-like MC3T3-E1 cells. 1992

R Okazaki, and K Ikeda, and A Sakamoto, and T Nakano, and K Morimoto, and T Kikuchi, and K Urakawa, and E Ogata, and T Matsumoto
Fourth Department of Internal Medicine, University of Tokyo Faculty of Medicine, Japan.

The present study was undertaken to clarify the relationship between c-fos and c-jun protooncogene expression and the differentiation and/or proliferation of osteoblasts, using osteoblast-like MC3T3-E1 (E1) cells. c-fos mRNA was barely detectable, whereas c-jun mRNA was constitutively expressed in E1 cells after serum deprivation for 24-72 h. When serum was added, a rapid and transient induction of c-fos and c-jun mRNAs was observed. The c-fos and c-jun mRNAs reached peak levels at 30 minutes, with a rapid disappearance of c-fos mRNA within 3 h and a much slower decrease in c-jun mRNA. The addition of serum together with cycloheximide, an inhibitor of protein synthesis, resulted in the superinduction of both c-fos and c-jun mRNAs. Among various growth factors, PDGF, EGF, and bFGF mimicked the serum effect, whereas IGF-I and TGF-beta failed to induce c-fos and c-jun mRNA. The effects of PDGF, EGF, and bFGF were completely abolished by pretreatment with actinomycin D, an inhibitor of RNA synthesis, suggesting a transcriptional mechanism. Nuclear runoff experiments showed that the transcription rate of c-fos and c-jun protooncogenes was increased by serum and growth factors. The effects of PDGF, EGF, and bFGF were inhibited by H-7 or staurosporine, inhibitors of protein kinase C (PKC), but not by HA1004 with a much weaker inhibitory activity, suggesting the involvement of PKC for the activation of the protooncogenes.(ABSTRACT TRUNCATED AT 250 WORDS)

UI MeSH Term Description Entries
D007334 Insulin-Like Growth Factor I A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on GROWTH HORMONE. It is believed to be mainly active in adults in contrast to INSULIN-LIKE GROWTH FACTOR II, which is a major fetal growth factor. IGF-I,Somatomedin C,IGF-1,IGF-I-SmC,Insulin Like Growth Factor I,Insulin-Like Somatomedin Peptide I,Insulin Like Somatomedin Peptide I
D007546 Isoquinolines A group of compounds with the heterocyclic ring structure of benzo(c)pyridine. The ring structure is characteristic of the group of opium alkaloids such as papaverine. (From Stedman, 25th ed)
D010006 Osteoblasts Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone. Osteoblast
D010879 Piperazines Compounds that are derived from PIPERAZINE.
D010982 Platelet-Derived Growth Factor Mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. Platelet Derived Growth Factor,Factor, Platelet-Derived Growth,Growth Factor, Platelet-Derived
D011519 Proto-Oncogenes Normal cellular genes homologous to viral oncogenes. The products of proto-oncogenes are important regulators of biological processes and appear to be involved in the events that serve to maintain the ordered procession through the cell cycle. Proto-oncogenes have names of the form c-onc. Proto-Oncogene,Proto Oncogene,Proto Oncogenes
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D003513 Cycloheximide Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis. Actidione,Cicloheximide
D003609 Dactinomycin A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015) Actinomycin,Actinomycin D,Meractinomycin,Cosmegen,Cosmegen Lyovac,Lyovac-Cosmegen,Lyovac Cosmegen,Lyovac, Cosmegen,LyovacCosmegen
D004815 Epidermal Growth Factor A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form. EGF,Epidermal Growth Factor-Urogastrone,Urogastrone,Human Urinary Gastric Inhibitor,beta-Urogastrone,Growth Factor, Epidermal,Growth Factor-Urogastrone, Epidermal,beta Urogastrone

Related Publications

R Okazaki, and K Ikeda, and A Sakamoto, and T Nakano, and K Morimoto, and T Kikuchi, and K Urakawa, and E Ogata, and T Matsumoto
Effects of microgravity on c-fos gene expression in osteoblast-like MC3T3-E1 cells.
January 1999, Advances in space research : the official journal of the Committee on Space Research (COSPAR),
R Okazaki, and K Ikeda, and A Sakamoto, and T Nakano, and K Morimoto, and T Kikuchi, and K Urakawa, and E Ogata, and T Matsumoto
C-fos expression of osteoblast-like MC3T3-E1 cells induced either by cooling or by fluid flow.
November 2004, Uchu Seibutsu Kagaku,
R Okazaki, and K Ikeda, and A Sakamoto, and T Nakano, and K Morimoto, and T Kikuchi, and K Urakawa, and E Ogata, and T Matsumoto
Activation of osteoblast-like MC3T3-E1 cell responses by poly(lactide).
December 2000, Biological & pharmaceutical bulletin,
R Okazaki, and K Ikeda, and A Sakamoto, and T Nakano, and K Morimoto, and T Kikuchi, and K Urakawa, and E Ogata, and T Matsumoto
Transcriptional regulation of alpha 2(I) collagen gene expression by fibroblast growth factor-2 in MC3T3-E1 osteoblast-like cells.
January 2001, Journal of cellular biochemistry,
R Okazaki, and K Ikeda, and A Sakamoto, and T Nakano, and K Morimoto, and T Kikuchi, and K Urakawa, and E Ogata, and T Matsumoto
Stimulation by bone sialoprotein of calcification in osteoblast-like MC3T3-E1 cells.
May 1995, Calcified tissue international,
R Okazaki, and K Ikeda, and A Sakamoto, and T Nakano, and K Morimoto, and T Kikuchi, and K Urakawa, and E Ogata, and T Matsumoto
Transcriptional activation of the mouse HSP47 gene in mouse osteoblast MC3T3-E1 cells by TGF-beta 1.
March 1998, Biochemical and biophysical research communications,
R Okazaki, and K Ikeda, and A Sakamoto, and T Nakano, and K Morimoto, and T Kikuchi, and K Urakawa, and E Ogata, and T Matsumoto
Mitogenic action of adenosine on osteoblast-like cells, MC3T3-E1.
May 1998, Calcified tissue international,
R Okazaki, and K Ikeda, and A Sakamoto, and T Nakano, and K Morimoto, and T Kikuchi, and K Urakawa, and E Ogata, and T Matsumoto
Fluoride-induced c-Fos expression in MC3T3-E1 osteoblastic cells.
February 2016, Toxicology mechanisms and methods,
R Okazaki, and K Ikeda, and A Sakamoto, and T Nakano, and K Morimoto, and T Kikuchi, and K Urakawa, and E Ogata, and T Matsumoto
Nephronectin expression is regulated by SMAD signaling in osteoblast-like MC3T3-E1 cells.
August 2012, Biochemical and biophysical research communications,
R Okazaki, and K Ikeda, and A Sakamoto, and T Nakano, and K Morimoto, and T Kikuchi, and K Urakawa, and E Ogata, and T Matsumoto
Translocation of Akt/PKB to the nucleus of osteoblast-like MC3T3-E1 cells exposed to proliferative growth factors.
July 2000, FEBS letters,
Copied contents to your clipboard!