Hyperthermia enhances tumor necrosis factor alpha-induced apoptosis of a human gastric cancer cell line. 2003

Satoshi Kokura, and Norimasa Yoshida, and Miho Ueda, and Eiko Imamoto, and Takeshi Ishikawa, and Tomohisa Takagi, and Yuji Naito, and Takeshi Okanoue, and Toshikazu Yoshikawa
Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. s-kokura@mpd.biglobe.ne.jp

We have investigated the effects of hyperthermia on the apoptosis induced by tumor necrosis factor alpha (TNF-alpha). Confluent monolayers of human gastric cancer cell line MKN45 were either treated or untreated with hyperthermia for 1 h. The cells were subsequently stimulated with TNF-alpha. A 24-h incubation with TNF-alpha did not affect cell viabilities; however, pretreatment with hyperthermia significantly enhanced the level of apoptosis induced by TNF-alpha. Pretreating MKN45 cells with hyperthermia (42.0 degrees C) significantly inhibited the TNF-alpha-induced increase in the binding activity of NF-kappaB to DNA. This study suggests that hyperthermia can inhibit the TNF-alpha-induced NF-kappaB activation and that hyperthermia renders human gastric cancer cells susceptible to the TNF-alpha-induced apoptosis, possibly via inhibition of the NF-kappaB pathway.

UI MeSH Term Description Entries
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D005334 Fever An abnormal elevation of body temperature, usually as a result of a pathologic process. Pyrexia,Fevers,Pyrexias
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013274 Stomach Neoplasms Tumors or cancer of the STOMACH. Cancer of Stomach,Gastric Cancer,Gastric Neoplasms,Stomach Cancer,Cancer of the Stomach,Gastric Cancer, Familial Diffuse,Neoplasms, Gastric,Neoplasms, Stomach,Cancer, Gastric,Cancer, Stomach,Cancers, Gastric,Cancers, Stomach,Gastric Cancers,Gastric Neoplasm,Neoplasm, Gastric,Neoplasm, Stomach,Stomach Cancers,Stomach Neoplasm
D014409 Tumor Necrosis Factor-alpha Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS. Cachectin,TNF-alpha,Tumor Necrosis Factor Ligand Superfamily Member 2,Cachectin-Tumor Necrosis Factor,TNF Superfamily, Member 2,TNFalpha,Tumor Necrosis Factor,Cachectin Tumor Necrosis Factor,Tumor Necrosis Factor alpha
D016328 NF-kappa B Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA. Immunoglobulin Enhancer-Binding Protein,NF-kappa B Complex,Nuclear Factor kappa B,Transcription Factor NF-kB,kappa B Enhancer Binding Protein,Ig-EBP-1,NF-kB,NF-kappaB,Nuclear Factor-Kappab,Complex, NF-kappa B,Enhancer-Binding Protein, Immunoglobulin,Factor NF-kB, Transcription,Factor-Kappab, Nuclear,Ig EBP 1,Immunoglobulin Enhancer Binding Protein,NF kB,NF kappa B Complex,NF kappaB,NF-kB, Transcription Factor,Nuclear Factor Kappab,Transcription Factor NF kB
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D053938 DNA Fragmentation Splitting the DNA into shorter pieces by endonucleolytic DNA CLEAVAGE at multiple sites. It includes the internucleosomal DNA fragmentation, which along with chromatin condensation, are considered to be the hallmarks of APOPTOSIS. DNA Degradation, Apoptotic,Apoptotic DNA Degradation,Fragmentation, DNA

Related Publications

Satoshi Kokura, and Norimasa Yoshida, and Miho Ueda, and Eiko Imamoto, and Takeshi Ishikawa, and Tomohisa Takagi, and Yuji Naito, and Takeshi Okanoue, and Toshikazu Yoshikawa
July 1999, Cancer research,
Satoshi Kokura, and Norimasa Yoshida, and Miho Ueda, and Eiko Imamoto, and Takeshi Ishikawa, and Tomohisa Takagi, and Yuji Naito, and Takeshi Okanoue, and Toshikazu Yoshikawa
November 1996, Experimental cell research,
Satoshi Kokura, and Norimasa Yoshida, and Miho Ueda, and Eiko Imamoto, and Takeshi Ishikawa, and Tomohisa Takagi, and Yuji Naito, and Takeshi Okanoue, and Toshikazu Yoshikawa
December 2006, International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group,
Satoshi Kokura, and Norimasa Yoshida, and Miho Ueda, and Eiko Imamoto, and Takeshi Ishikawa, and Tomohisa Takagi, and Yuji Naito, and Takeshi Okanoue, and Toshikazu Yoshikawa
November 2004, Cell death and differentiation,
Satoshi Kokura, and Norimasa Yoshida, and Miho Ueda, and Eiko Imamoto, and Takeshi Ishikawa, and Tomohisa Takagi, and Yuji Naito, and Takeshi Okanoue, and Toshikazu Yoshikawa
June 2013, Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery,
Satoshi Kokura, and Norimasa Yoshida, and Miho Ueda, and Eiko Imamoto, and Takeshi Ishikawa, and Tomohisa Takagi, and Yuji Naito, and Takeshi Okanoue, and Toshikazu Yoshikawa
May 2004, Immunopharmacology and immunotoxicology,
Satoshi Kokura, and Norimasa Yoshida, and Miho Ueda, and Eiko Imamoto, and Takeshi Ishikawa, and Tomohisa Takagi, and Yuji Naito, and Takeshi Okanoue, and Toshikazu Yoshikawa
December 2014, World journal of gastroenterology,
Satoshi Kokura, and Norimasa Yoshida, and Miho Ueda, and Eiko Imamoto, and Takeshi Ishikawa, and Tomohisa Takagi, and Yuji Naito, and Takeshi Okanoue, and Toshikazu Yoshikawa
April 2000, Oncogene,
Satoshi Kokura, and Norimasa Yoshida, and Miho Ueda, and Eiko Imamoto, and Takeshi Ishikawa, and Tomohisa Takagi, and Yuji Naito, and Takeshi Okanoue, and Toshikazu Yoshikawa
July 2005, Cancer research,
Satoshi Kokura, and Norimasa Yoshida, and Miho Ueda, and Eiko Imamoto, and Takeshi Ishikawa, and Tomohisa Takagi, and Yuji Naito, and Takeshi Okanoue, and Toshikazu Yoshikawa
October 2004, Oncogene,
Copied contents to your clipboard!