Biomaterial Database

Carnitine profiles during differentiation and effects of carnitine on differentiation of 3T3-L1 cells. 2003

Youn-Soo Cha, and Jae-Soon Eun, and Suk-Heung Oh

Department of Food Science and Human Nutrition and Institute for Molecular Biology and Genetics, Chonbuk National University, Korea. cha8@moak.chonbuk.ac.kr

These experiments were conducted to investigate carnitine profiles during differentiation and the effects of carnitine on the differentiation of 3T3-L1 cells. To induce cell differentiation, undifferentiated 3T3-L1 cells were treated with dexamethasone, 1-methyl-3-isobutylxanthine, and d-biotin. Carnitine was also exogenously added to the cells to test its effect on cell differentiation. Triglyceride, total lipid, total protein, nonesterified carnitine, acid-soluble acylcarnitine, and acid-insoluble acylcarnitine were analyzed during the differentiation of 3T3-L1 cells. Total lipid, triglyceride, and total protein increased during the 3T3-L1 cell differentiation. However, nonesterified carnitine, acid-soluble acylcarnitine, and acid-insoluble acylcarnitine concentrations were lower in the differentiated 3T3-L1 cells. In addition, the exogenously added carnitine inhibited the increases in triglyceride and total lipid levels. These results suggest that carnitine may have an inhibitory role on the early stage of 3T3-L1 cell differentiation.

UI	MeSH Term	Description	Entries
D011506	Proteins	Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.	Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D002331	Carnitine	A constituent of STRIATED MUSCLE and LIVER. It is an amino acid derivative and an essential cofactor for fatty acid metabolism.	Bicarnesine,L-Carnitine,Levocarnitine,Vitamin BT,L Carnitine
D002454	Cell Differentiation	Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.	Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014280	Triglycerides	An ester formed from GLYCEROL and three fatty acid groups.	Triacylglycerol,Triacylglycerols,Triglyceride
D050356	Lipid Metabolism	Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.	Metabolism, Lipid
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D041721	3T3-L1 Cells	A continuous cell line that is a substrain of SWISS 3T3 CELLS developed though clonal isolation. The mouse fibroblast cells undergo an adipose-like conversion as they move to a confluent and contact-inhibited state.	3T3-L1,3T3 L1 Cells,3T3-L1 Cell,Cell, 3T3-L1,Cells, 3T3-L1