BIOMAT Database Logo BIOMAT Database Logo

Protein structural effects of agonist binding to the nicotinic acetylcholine receptor. 1992

J Castresana, and G Fernandez-Ballester, and A M Fernandez, and J L Laynez, and J L Arrondo, and J A Ferragut, and J M Gonzalez-Ros
Department of Neurochemistry, University of Alicante, Spain.

The effects on the protein structure produced by binding of cholinergic agonists to purified acetylcholine receptor (AcChR) reconstituted into lipid vesicles, has been studied by Fourier-transform infrared spectroscopy and differential scanning calorimetry. Spectral changes in the conformationally sensitive amide I infrared band indicates that the exposure of the AcChR to the agonist carbamylcholine, under conditions which drive the AcChR into the desensitized state, produces alterations in the protein secondary structure. Quantitative estimation of these agonist-induced alterations by band-fitting analysis of the amide I spectral band reveals no appreciable changes in the percent of alpha-helix, but a decrease in beta-sheet structure, concomitant with an increase in less ordered structures. Additionally, agonist binding results in a concentration-dependent increase in the protein thermal stability, as indicated by the temperature dependence of the protein infrared spectrum and by calorimetric analysis, which further suggest that AcChR desensitization induced by the cholinergic agonist implies significant rearrangements in the protein structure.

UI MeSH Term Description Entries
D011978 Receptors, Nicotinic One of the two major classes of cholinergic receptors. Nicotinic receptors were originally distinguished by their preference for NICOTINE over MUSCARINE. They are generally divided into muscle-type and neuronal-type (previously ganglionic) based on pharmacology, and subunit composition of the receptors. Nicotinic Acetylcholine Receptors,Nicotinic Receptors,Nicotinic Acetylcholine Receptor,Nicotinic Receptor,Acetylcholine Receptor, Nicotinic,Acetylcholine Receptors, Nicotinic,Receptor, Nicotinic,Receptor, Nicotinic Acetylcholine,Receptors, Nicotinic Acetylcholine
D002152 Calorimetry, Differential Scanning Differential thermal analysis in which the sample compartment of the apparatus is a differential calorimeter, allowing an exact measure of the heat of transition independent of the specific heat, thermal conductivity, and other variables of the sample. Differential Thermal Analysis, Calorimetric,Calorimetric Differential Thermal Analysis,Differential Scanning Calorimetry,Scanning Calorimetry, Differential
D002217 Carbachol A slowly hydrolyzed CHOLINERGIC AGONIST that acts at both MUSCARINIC RECEPTORS and NICOTINIC RECEPTORS. Carbamylcholine,Carbacholine,Carbamann,Carbamoylcholine,Carbastat,Carbocholine,Carboptic,Doryl,Isopto Carbachol,Jestryl,Miostat,Carbachol, Isopto
D005583 Fourier Analysis Analysis based on the mathematical function first formulated by Jean-Baptiste-Joseph Fourier in 1807. The function, known as the Fourier transform, describes the sinusoidal pattern of any fluctuating pattern in the physical world in terms of its amplitude and its phase. It has broad applications in biomedicine, e.g., analysis of the x-ray crystallography data pivotal in identifying the double helical nature of DNA and in analysis of other molecules, including viruses, and the modified back-projection algorithm universally used in computerized tomography imaging, etc. (From Segen, The Dictionary of Modern Medicine, 1992) Fourier Series,Fourier Transform,Analysis, Cyclic,Analysis, Fourier,Cyclic Analysis,Analyses, Cyclic,Cyclic Analyses,Series, Fourier,Transform, Fourier
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013055 Spectrophotometry, Infrared Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) IR Spectra,Infrared Spectrophotometry,IR Spectras,Spectra, IR
D014101 Torpedo A genus of the Torpedinidae family consisting of several species. Members of this family have powerful electric organs and are commonly called electric rays. Electric Rays,Torpedinidae,Rays, Electric
D017433 Protein Structure, Secondary The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to ALPHA-HELICES; BETA-STRANDS (which align to form BETA-SHEETS), or other types of coils. This is the first folding level of protein conformation. Secondary Protein Structure,Protein Structures, Secondary,Secondary Protein Structures,Structure, Secondary Protein,Structures, Secondary Protein

Related Publications

J Castresana, and G Fernandez-Ballester, and A M Fernandez, and J L Laynez, and J L Arrondo, and J A Ferragut, and J M Gonzalez-Ros
Agonist self-inhibitory binding site of the nicotinic acetylcholine receptor.
April 1996, Journal of neuroscience research,
J Castresana, and G Fernandez-Ballester, and A M Fernandez, and J L Laynez, and J L Arrondo, and J A Ferragut, and J M Gonzalez-Ros
Agonist binding to the nicotinic acetylcholine receptor and probability of channel opening.
January 1988, Neurochemistry international,
J Castresana, and G Fernandez-Ballester, and A M Fernandez, and J L Laynez, and J L Arrondo, and J A Ferragut, and J M Gonzalez-Ros
Effects of antibody binding on structural transitions of the nicotinic acetylcholine receptor.
September 1996, Biochemistry,
J Castresana, and G Fernandez-Ballester, and A M Fernandez, and J L Laynez, and J L Arrondo, and J A Ferragut, and J M Gonzalez-Ros
Nicotinic acetylcholine receptor diversity: agonist binding and functional potency.
January 1989, Progress in brain research,
J Castresana, and G Fernandez-Ballester, and A M Fernandez, and J L Laynez, and J L Arrondo, and J A Ferragut, and J M Gonzalez-Ros
Site-selective agonist binding to the nicotinic acetylcholine receptor from Torpedo californica.
April 2003, Biochemistry,
J Castresana, and G Fernandez-Ballester, and A M Fernandez, and J L Laynez, and J L Arrondo, and J A Ferragut, and J M Gonzalez-Ros
Roles of agonist-binding sites in nicotinic acetylcholine receptor function.
December 2000, Biochemical and biophysical research communications,
J Castresana, and G Fernandez-Ballester, and A M Fernandez, and J L Laynez, and J L Arrondo, and J A Ferragut, and J M Gonzalez-Ros
Photoactivatable agonist of the nicotinic acetylcholine receptor: potential probe to characterize the structural transitions of the acetylcholine binding site in different states of the receptor.
June 1992, Molecular pharmacology,
J Castresana, and G Fernandez-Ballester, and A M Fernandez, and J L Laynez, and J L Arrondo, and J A Ferragut, and J M Gonzalez-Ros
A structural model of agonist binding to the alpha3beta4 neuronal nicotinic receptor.
November 2003, British journal of pharmacology,
J Castresana, and G Fernandez-Ballester, and A M Fernandez, and J L Laynez, and J L Arrondo, and J A Ferragut, and J M Gonzalez-Ros
The tethered agonist approach to mapping ion channel proteins--toward a structural model for the agonist binding site of the nicotinic acetylcholine receptor.
January 2001, Chemistry & biology,
J Castresana, and G Fernandez-Ballester, and A M Fernandez, and J L Laynez, and J L Arrondo, and J A Ferragut, and J M Gonzalez-Ros
Structural heterogeneity of the alpha subunits of the nicotinic acetylcholine receptor in relation to agonist affinity alkylation and antagonist binding.
July 1986, Biochemistry,
Copied contents to your clipboard!