BIOMAT Database Logo BIOMAT Database Logo

Determination of aristolochic acids by capillary electrophoresis. 2003

Horacio A Priestap, and Silvia L Iglesias, and Martín F Desimone, and Luis E Diaz
Cátedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

A method for the separation and quantification of aristolochic acids by capillary electrophoresis is described. Buffer solutions composed of sodium dihydrogen phosphate, sodium borate, and sodium dodecyl sulfate at pH 6.5-7 were found to be suitable for the separation of aristolochic acids, which can be well resolved in a few minutes. The separation and identification of six aristolochic acids contained in a commercial tincture called charrua, a traditional herbal medicine consisting of a hydroalcoholic extract of Aristolochia argentina, corroborate the usefulness of the method. The eventual toxic properties of the aristolochic acid containing charrua tincture are discussed.

UI MeSH Term Description Entries
D010936 Plant Extracts Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard. Herbal Medicines,Plant Extract,Extract, Plant,Extracts, Plant,Medicines, Herbal
D010946 Plants, Medicinal Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals. Herbs, Medicinal,Medicinal Herbs,Healing Plants,Medicinal Plants,Pharmaceutical Plants,Healing Plant,Herb, Medicinal,Medicinal Herb,Medicinal Plant,Pharmaceutical Plant,Plant, Healing,Plant, Medicinal,Plant, Pharmaceutical,Plants, Healing,Plants, Pharmaceutical
D019075 Electrophoresis, Capillary A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992) Capillary Zone Electrophoresis,Capillary Electrophoreses,Capillary Electrophoresis,Capillary Zone Electrophoreses,Electrophoreses, Capillary,Electrophoreses, Capillary Zone,Electrophoresis, Capillary Zone,Zone Electrophoreses, Capillary,Zone Electrophoresis, Capillary
D030022 Aristolochia A plant genus of the family ARISTOLOCHIACEAE. Species of this genus have been used in traditional medicine but they contain aristolochic acid which is associated with nephropathy. These are sometimes called 'snakeroot' but that name is also used with a number of other plants such as POLYGALA; SANICULA; ASARUM; ARISTOLOCHIA; AGERATINA; and others. Birthwort,Dutchman's Pipe,Aristolochias,Birthworts,Dutchman Pipe,Dutchman's Pipes,Dutchmans Pipe,Pipe, Dutchman's,Pipes, Dutchman's
D034341 Aristolochic Acids Nitro-phenanthrenes occurring in ARISTOLOCHIACEAE and other plants. They derive from stephanine (APORPHINES) by oxidative ring cleavage. The nitro group is a reactive alkylator (ALKYLATING AGENTS) that binds to biological macromolecules. Ingestion by humans is associated with nephropathy (NEPHRITIS). There is no relationship to the similar named aristolochene (SESQUITERPENES).

Related Publications

Horacio A Priestap, and Silvia L Iglesias, and Martín F Desimone, and Luis E Diaz
Determination of aristolochic acids in medicinal plants (Chinese) prepared medicine using capillary zone electrophoresis.
July 2001, Electrophoresis,
Horacio A Priestap, and Silvia L Iglesias, and Martín F Desimone, and Luis E Diaz
Determination of dissociation constants of aristolochic acid I and II by capillary electrophoresis with carboxymethyl chitosan-coated capillary.
July 2011, Talanta,
Horacio A Priestap, and Silvia L Iglesias, and Martín F Desimone, and Luis E Diaz
Determination of organic acids in urine by capillary zone electrophoresis.
September 1994, Journal of chromatography. A,
Horacio A Priestap, and Silvia L Iglesias, and Martín F Desimone, and Luis E Diaz
Determination of phenolic acids in olive oil by capillary electrophoresis.
January 2004, Annali di chimica,
Horacio A Priestap, and Silvia L Iglesias, and Martín F Desimone, and Luis E Diaz
Determination of alpha hydroxy acids in fruits by capillary electrophoresis.
August 2002, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
Horacio A Priestap, and Silvia L Iglesias, and Martín F Desimone, and Luis E Diaz
Determination of aristolochic acid in Chinese herbal medicine by capillary electrophoresis with laser-induced fluorescence detection.
February 2006, Journal of chromatography. A,
Horacio A Priestap, and Silvia L Iglesias, and Martín F Desimone, and Luis E Diaz
Determination of dissociation constants of amino acids by capillary zone electrophoresis.
October 1999, Electrophoresis,
Horacio A Priestap, and Silvia L Iglesias, and Martín F Desimone, and Luis E Diaz
Determination of alpha-hydroxy acids in cosmetic products by capillary electrophoresis.
February 2006, Journal of pharmaceutical and biomedical analysis,
Horacio A Priestap, and Silvia L Iglesias, and Martín F Desimone, and Luis E Diaz
Determination of amino acids in rat vitreous perfusates by capillary electrophoresis.
September 2004, Electrophoresis,
Horacio A Priestap, and Silvia L Iglesias, and Martín F Desimone, and Luis E Diaz
Determination of organic acids in food samples by capillary zone electrophoresis.
June 2000, Journal of chromatography. A,
Copied contents to your clipboard!