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The aim of this study was to investigate the role of immunoglobulin E (IgE) in the late phase reaction (LPR) of murine experimental asthma. Our model consisted of an implant of DNP-conjugated, heat-coagulated hen's egg white (DNP-EWI), followed 14 days later by an intratracheal challenge with aggregated DNP-ovalbumin. Airway inflammation was analyzed 48 h after challenge and compared with a similarly immunized group of mice with highly suppressed humoral response due to anti-micro and anti-delta antibody treatment. Total number of cells in the bronchoalveolar lavage (BAL) (with predominance of eosinophils) and EPO activity in the lung homogenate were increased in the DNP-EWI-immunized group compared with immunosuppressed or nonimmunized mice. However, the cellular infiltration and EPO activity observed in the immunosuppressed group were still significantly above those obtained in the nonimmunized group, indicating that inhibition of antibody production did not completely prevent the inflammatory manifestations in BAL and lung. Airway hyperresponsiveness to methacoline was obtained in DNP-EWI-immunized mice, but the respiratory mechanical parameters returned to normal levels in the immunosuppressed group. When these mice were reconstituted with monoclonal anti-DNP antibodies, only IgE, but not IgG1, restored lung inflammation and decreased the conductance of the respiratory system, therefore, increasing hyperresponsiveness. These results indicate that antibodies are not essential for induction of LPR in the lung. However, IgE enhances pulmonary inflammation and hyperresponsiveness.
D M Itami, and
D Latinne, and
H Bazin, and
M L B Garcia, and
A Perini, and
M A Martins, and
M S Macedo, and
M F Macedo-Soares
September 2000,
American journal of respiratory cell and molecular biology,
D M Itami, and
D Latinne, and
H Bazin, and
M L B Garcia, and
A Perini, and
M A Martins, and
M S Macedo, and
M F Macedo-Soares
October 1999,
American journal of respiratory cell and molecular biology,
D M Itami, and
D Latinne, and
H Bazin, and
M L B Garcia, and
A Perini, and
M A Martins, and
M S Macedo, and
M F Macedo-Soares
June 2001,
The Journal of allergy and clinical immunology,
D M Itami, and
D Latinne, and
H Bazin, and
M L B Garcia, and
A Perini, and
M A Martins, and
M S Macedo, and
M F Macedo-Soares
October 2013,
Respiratory research,
D M Itami, and
D Latinne, and
H Bazin, and
M L B Garcia, and
A Perini, and
M A Martins, and
M S Macedo, and
M F Macedo-Soares
September 2014,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
D M Itami, and
D Latinne, and
H Bazin, and
M L B Garcia, and
A Perini, and
M A Martins, and
M S Macedo, and
M F Macedo-Soares
September 1999,
American journal of respiratory and critical care medicine,
D M Itami, and
D Latinne, and
H Bazin, and
M L B Garcia, and
A Perini, and
M A Martins, and
M S Macedo, and
M F Macedo-Soares
June 1999,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
D M Itami, and
D Latinne, and
H Bazin, and
M L B Garcia, and
A Perini, and
M A Martins, and
M S Macedo, and
M F Macedo-Soares
July 1998,
Pediatric pulmonology,
D M Itami, and
D Latinne, and
H Bazin, and
M L B Garcia, and
A Perini, and
M A Martins, and
M S Macedo, and
M F Macedo-Soares
February 2015,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
D M Itami, and
D Latinne, and
H Bazin, and
M L B Garcia, and
A Perini, and
M A Martins, and
M S Macedo, and
M F Macedo-Soares
April 1987,
Journal of applied physiology (Bethesda, Md. : 1985),
