Characterization of activated and normal mouse Mos gene in murine 3T3 cells. 1992

R S Paules, and J Resnick, and A B Kasenally, and M K Ernst, and P Donovan, and G F Vande Woude
Mammalian Molecular Genetics Group, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.

We have characterized the mouse Mos proto-oncogene product, pp39Mos, in murine fibroblasts. When expressed in NIH3T3 cells under the influence of the long terminal repeat regulatory element from Moloney murine sarcoma virus [NIH(pTS-1) cells], the Mos protein was present in low levels and had a half-life of about 30 min. In extracts from NIH(pTS-1) cells, we detected additional forms of Mos protein that apparently arose from internal initiation codons (p24Mos and p29Mos) or from upstream non-AUG initiation codons (p42Mos and p44Mos). The Mos protein was found to exist in these cells as a phosphoprotein, pp39Mos, and, when immunoprecipitated with an antiserum specific for the Mos N-terminus [anti-Mos(6-24)], had autophosphorylating kinase activity. We found that anti-Mos(6-24) also detected non-Mos protein kinase activity and non-Mos phosphoproteins in addition to p39Mos. We present evidence, on both the RNA and protein levels, that non-transformed mouse 3T3 cells do not express endogenous Mos.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008715 Methionine A sulfur-containing essential L-amino acid that is important in many body functions. L-Methionine,Liquimeth,Methionine, L-Isomer,Pedameth,L-Isomer Methionine,Methionine, L Isomer
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D008980 Moloney murine sarcoma virus A replication-defective murine sarcoma virus (SARCOMA VIRUSES, MURINE) isolated from a rhabdomyosarcoma by Moloney in 1966. Moloney Sarcoma Virus,Sarcoma Virus, Moloney,Virus, Moloney Sarcoma
D010750 Phosphoproteins Phosphoprotein
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D011505 Protein-Tyrosine Kinases Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors. Tyrosine Protein Kinase,Tyrosine-Specific Protein Kinase,Protein-Tyrosine Kinase,Tyrosine Kinase,Tyrosine Protein Kinases,Tyrosine-Specific Protein Kinases,Tyrosylprotein Kinase,Kinase, Protein-Tyrosine,Kinase, Tyrosine,Kinase, Tyrosine Protein,Kinase, Tyrosine-Specific Protein,Kinase, Tyrosylprotein,Kinases, Protein-Tyrosine,Kinases, Tyrosine Protein,Kinases, Tyrosine-Specific Protein,Protein Kinase, Tyrosine-Specific,Protein Kinases, Tyrosine,Protein Kinases, Tyrosine-Specific,Protein Tyrosine Kinase,Protein Tyrosine Kinases,Tyrosine Specific Protein Kinase,Tyrosine Specific Protein Kinases
D012045 Regulatory Sequences, Nucleic Acid Nucleic acid sequences involved in regulating the expression of genes. Nucleic Acid Regulatory Sequences,Regulatory Regions, Nucleic Acid (Genetics),Region, Regulatory,Regions, Regulatory,Regulator Regions, Nucleic Acid,Regulatory Region,Regulatory Regions
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D003062 Codon A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE). Codon, Sense,Sense Codon,Codons,Codons, Sense,Sense Codons

Related Publications

R S Paules, and J Resnick, and A B Kasenally, and M K Ernst, and P Donovan, and G F Vande Woude
November 1976, Journal of cellular physiology,
R S Paules, and J Resnick, and A B Kasenally, and M K Ernst, and P Donovan, and G F Vande Woude
March 1989, Journal of virology,
R S Paules, and J Resnick, and A B Kasenally, and M K Ernst, and P Donovan, and G F Vande Woude
July 1973, Nature: New biology,
R S Paules, and J Resnick, and A B Kasenally, and M K Ernst, and P Donovan, and G F Vande Woude
June 1987, Biochemistry,
R S Paules, and J Resnick, and A B Kasenally, and M K Ernst, and P Donovan, and G F Vande Woude
October 1982, Virology,
R S Paules, and J Resnick, and A B Kasenally, and M K Ernst, and P Donovan, and G F Vande Woude
February 1982, Biochemical and biophysical research communications,
R S Paules, and J Resnick, and A B Kasenally, and M K Ernst, and P Donovan, and G F Vande Woude
July 1977, Virology,
R S Paules, and J Resnick, and A B Kasenally, and M K Ernst, and P Donovan, and G F Vande Woude
April 2006, Gene,
R S Paules, and J Resnick, and A B Kasenally, and M K Ernst, and P Donovan, and G F Vande Woude
December 1986, Cellular immunology,
R S Paules, and J Resnick, and A B Kasenally, and M K Ernst, and P Donovan, and G F Vande Woude
November 1988, Proceedings of the National Academy of Sciences of the United States of America,
Copied contents to your clipboard!