[Determination of benzodiazepine derivatives mixture by high performance liquid chromatography]. 2003

Andrejus Zevzikovas, and Augusta Bertulyte, and Vidmantas Dirse, and Liudas Ivanauskas
Department of Analytical and Toxicological Chemistry, Kaunas University of Medicine, Kaunas, Lithuania. andrejuszev@email.lt

By increasing tendency of intoxication with several preparations at a time, there is lack of data in the sources of information about the chemical-toxicological research of the psychotropic drugs in the mixture. The aim of this research was to determine possibility of qualitative analysis of benzodiazepines derivatives--diazepam, flunitrazepam, nitrazepam, oxazepam and tetrazepam in the mixture, extracted from biological fluid (blood). The high performance liquid chromatography was used for the investigation, the reagents--tablets of diazepam, flunitrazepam, nitrazepam, oxazepam and tetrazepam. It was found out that the most acceptable eliuent for the analysis of the mixture by high pressure liquid chromatography is phosphate buffer, containing 0.02 M penthanesulfonic acid:etannitrile (55:45) (pH=3.5). The optimal wavelength for identification of the drugs in the mixture is 254 nm.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D002021 Buffers A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer. Buffer
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001569 Benzodiazepines A group of two-ring heterocyclic compounds consisting of a benzene ring fused to a diazepine ring. Benzodiazepine,Benzodiazepine Compounds
D013607 Tablets Solid dosage forms, of varying weight, size, and shape, which may be molded or compressed, and which contain a medicinal substance in pure or diluted form. (Dorland, 28th ed) Tablet
D014151 Anti-Anxiety Agents Agents that alleviate ANXIETY, tension, and ANXIETY DISORDERS, promote sedation, and have a calming effect without affecting clarity of consciousness or neurologic conditions. ADRENERGIC BETA-ANTAGONISTS are commonly used in the symptomatic treatment of anxiety but are not included here. Anti-Anxiety Agent,Anti-Anxiety Drug,Anxiolytic,Anxiolytic Agent,Anxiolytic Agents,Tranquilizing Agents, Minor,Anti-Anxiety Drugs,Anti-Anxiety Effect,Anti-Anxiety Effects,Antianxiety Effect,Antianxiety Effects,Anxiolytic Effect,Anxiolytic Effects,Anxiolytics,Tranquillizing Agents, Minor,Agent, Anti-Anxiety,Agent, Anxiolytic,Agents, Anti-Anxiety,Agents, Anxiolytic,Agents, Minor Tranquilizing,Agents, Minor Tranquillizing,Anti Anxiety Agent,Anti Anxiety Agents,Anti Anxiety Drug,Anti Anxiety Drugs,Anti Anxiety Effect,Anti Anxiety Effects,Drug, Anti-Anxiety,Drugs, Anti-Anxiety,Effect, Anti-Anxiety,Effect, Antianxiety,Effect, Anxiolytic,Effects, Anti-Anxiety,Effects, Antianxiety,Effects, Anxiolytic,Minor Tranquilizing Agents,Minor Tranquillizing Agents

Related Publications

Andrejus Zevzikovas, and Augusta Bertulyte, and Vidmantas Dirse, and Liudas Ivanauskas
June 1999, Journal of chromatography. A,
Andrejus Zevzikovas, and Augusta Bertulyte, and Vidmantas Dirse, and Liudas Ivanauskas
January 2003, Medicina (Kaunas, Lithuania),
Andrejus Zevzikovas, and Augusta Bertulyte, and Vidmantas Dirse, and Liudas Ivanauskas
April 1978, Analytical chemistry,
Andrejus Zevzikovas, and Augusta Bertulyte, and Vidmantas Dirse, and Liudas Ivanauskas
June 1996, Journal of pharmaceutical and biomedical analysis,
Andrejus Zevzikovas, and Augusta Bertulyte, and Vidmantas Dirse, and Liudas Ivanauskas
January 1981, Journal of agricultural and food chemistry,
Andrejus Zevzikovas, and Augusta Bertulyte, and Vidmantas Dirse, and Liudas Ivanauskas
January 2002, Medicina (Kaunas, Lithuania),
Andrejus Zevzikovas, and Augusta Bertulyte, and Vidmantas Dirse, and Liudas Ivanauskas
January 1989, Journal of pharmaceutical and biomedical analysis,
Andrejus Zevzikovas, and Augusta Bertulyte, and Vidmantas Dirse, and Liudas Ivanauskas
April 1991, Journal of chromatography,
Andrejus Zevzikovas, and Augusta Bertulyte, and Vidmantas Dirse, and Liudas Ivanauskas
December 1984, Journal of chromatography,
Andrejus Zevzikovas, and Augusta Bertulyte, and Vidmantas Dirse, and Liudas Ivanauskas
November 1984, Journal of chromatography,
Copied contents to your clipboard!