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We have measured the in vivo unidirectional rates between the terminal phosphate of ATP and intracellular inorganic phosphate (PiIN) in aerobic suspensions of Escherichia coli cells using 31P nuclear magnetic resonance saturation transfer techniques. Typically, the measurements consisted of saturating the ATPgamma resonance and observing a 20 +/- 5% reduction in the intensity of the PiIN resonance. No saturation transfer was observed after incubation with 1 mM dicyclohexylcarbodiimide, an ATPase inhibitor. From the measured decrease in intensity of PiIN coming from saturation transfer, the apparent unimolecular rate constant of PiIN to ATP was calculated to be 0.6 +/- 0.15 sec-1.
T R Brown, and
K Ugurbil, and
R G Shulman
January 1989,
Advances in experimental medicine and biology,
T R Brown, and
K Ugurbil, and
R G Shulman
March 1977,
Proceedings of the National Academy of Sciences of the United States of America,
T R Brown, and
K Ugurbil, and
R G Shulman
May 1978,
Proceedings of the National Academy of Sciences of the United States of America,
T R Brown, and
K Ugurbil, and
R G Shulman
December 2004,
Applied and environmental microbiology,
T R Brown, and
K Ugurbil, and
R G Shulman
April 1978,
Biochimica et biophysica acta,
T R Brown, and
K Ugurbil, and
R G Shulman
September 1977,
Proceedings of the National Academy of Sciences of the United States of America,
T R Brown, and
K Ugurbil, and
R G Shulman
October 1981,
Proceedings of the National Academy of Sciences of the United States of America,
T R Brown, and
K Ugurbil, and
R G Shulman
April 1999,
European journal of biochemistry,
T R Brown, and
K Ugurbil, and
R G Shulman
November 1977,
Proceedings of the National Academy of Sciences of the United States of America,
T R Brown, and
K Ugurbil, and
R G Shulman
April 1988,
Biochimica et biophysica acta,
