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Eosinophils contain four principal cationic proteins, major basic protein (MBP), eosinophil-derived neurotoxin (EDN), eosinophil cationic protein (ECP), and eosinophil peroxidase (EPO). To determine the quantities of these proteins in granulocytes and whether they are specific to eosinophils, their concentrations in lysates of human granulocytes were measured using specific radioimmunoassays. The effect of different methods for eosinophil lysis on the recovery of the proteins was also studied. Maximal recovery occurred at pH 2 for MBP and pH 5.6 for the other granule proteins. The proteins cosedimented with eosinophils and their concentrations (mean +/- SEM) in ng/10(6) eosinophils (and in nM/10(6) eosinophils) were: MBP, 8,982 +/- 611 (641.6); EDN, 3,283 +/- 116 (178.4); ECP, 5,269 +/- 283 (250.9); and EPO, 12,174 +/- 859 (171.5). Basophils from a normal person contained (in ng/10(6) cells) MBP, 2,374; EDN, 214; ECP, 77; and EPO, 17. Highly purified neutrophils contained (in ng/10(6) cells) MBP, 3 +/- 0.5; EDN, 72 +/- 9; and ECP, 50 +/- 12. Therefore we conclude that these proteins are mainly expressed in eosinophils, but that certain ones are present in basophils and neutrophils.
R I Abu-Ghazaleh, and
S L Dunnette, and
D A Loegering, and
J L Checkel, and
H Kita, and
L L Thomas, and
G J Gleich
September 1999,
Nihon rinsho. Japanese journal of clinical medicine,
R I Abu-Ghazaleh, and
S L Dunnette, and
D A Loegering, and
J L Checkel, and
H Kita, and
L L Thomas, and
G J Gleich
May 2006,
La Revue de medecine interne,
R I Abu-Ghazaleh, and
S L Dunnette, and
D A Loegering, and
J L Checkel, and
H Kita, and
L L Thomas, and
G J Gleich
May 1993,
Blood,
R I Abu-Ghazaleh, and
S L Dunnette, and
D A Loegering, and
J L Checkel, and
H Kita, and
L L Thomas, and
G J Gleich
January 1993,
Agents and actions. Supplements,
R I Abu-Ghazaleh, and
S L Dunnette, and
D A Loegering, and
J L Checkel, and
H Kita, and
L L Thomas, and
G J Gleich
December 2005,
Journal of immunological methods,
R I Abu-Ghazaleh, and
S L Dunnette, and
D A Loegering, and
J L Checkel, and
H Kita, and
L L Thomas, and
G J Gleich
March 1993,
Nihon rinsho. Japanese journal of clinical medicine,
R I Abu-Ghazaleh, and
S L Dunnette, and
D A Loegering, and
J L Checkel, and
H Kita, and
L L Thomas, and
G J Gleich
June 2014,
The Journal of biological chemistry,
R I Abu-Ghazaleh, and
S L Dunnette, and
D A Loegering, and
J L Checkel, and
H Kita, and
L L Thomas, and
G J Gleich
April 1978,
British journal of haematology,
R I Abu-Ghazaleh, and
S L Dunnette, and
D A Loegering, and
J L Checkel, and
H Kita, and
L L Thomas, and
G J Gleich
October 1990,
The Journal of experimental medicine,
R I Abu-Ghazaleh, and
S L Dunnette, and
D A Loegering, and
J L Checkel, and
H Kita, and
L L Thomas, and
G J Gleich
January 2000,
Chemical immunology,
