BIOMAT Database Logo BIOMAT Database Logo

p19ARF-induced p53-independent apoptosis largely occurs through BAX. 2003

Hiroaki Suzuki, and Megumi Kurita, and Kiyohisa Mizumoto, and Ikuo Nishimoto, and Etsuro Ogata, and Masaaki Matsuoka
Department of Pharmacology, KEIO University School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160-8582, Tokyo, Japan.

Combined disruption of the ARF gene and the p53 gene causes mouse predisposition to tumors of a wider variety and at a higher frequency than disruption of the p53 gene, indicating that the ARF gene has p53-independent anti-tumor function in addition to p53-dependent function. Coincidentally with this notion, ectopic expression of the p19(ARF) induces apoptosis for wild-type mouse embryo fibroblasts which have been immortalized by introduction of the SV40 virus genome (SV40-MEFs). The protein expression levels of p53, p21(Cip1), and Bax were not upregulated by ectopic expression of p19(ARF) in SV40-MEFs, indicating that expression of p19(ARF) induced apoptosis through p53-independent pathways in this system. Ectopic expression of p19(ARF) induced prominent apoptosis even in SV40-Bak-/-MEFs. In contrast, expression of p19(ARF) induced only a very low grade of apoptosis in Bax-/- or Bax-/-/Bak-/-SV40-MEFs. Remarkable attenuation of p19(ARF)-induced apoptosis by disruption of the Bax gene thus leads to the conclusion that Bax plays a major role in p53-independent apoptosis induced by p19(ARF).

UI MeSH Term Description Entries
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D008565 Membrane Proteins Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors. Cell Membrane Protein,Cell Membrane Proteins,Cell Surface Protein,Cell Surface Proteins,Integral Membrane Proteins,Membrane-Associated Protein,Surface Protein,Surface Proteins,Integral Membrane Protein,Membrane Protein,Membrane-Associated Proteins,Membrane Associated Protein,Membrane Associated Proteins,Membrane Protein, Cell,Membrane Protein, Integral,Membrane Proteins, Integral,Protein, Cell Membrane,Protein, Cell Surface,Protein, Integral Membrane,Protein, Membrane,Protein, Membrane-Associated,Protein, Surface,Proteins, Cell Membrane,Proteins, Cell Surface,Proteins, Integral Membrane,Proteins, Membrane,Proteins, Membrane-Associated,Proteins, Surface,Surface Protein, Cell
D011518 Proto-Oncogene Proteins Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity. Cellular Proto-Oncogene Proteins,c-onc Proteins,Proto Oncogene Proteins, Cellular,Proto-Oncogene Products, Cellular,Cellular Proto Oncogene Proteins,Cellular Proto-Oncogene Products,Proto Oncogene Products, Cellular,Proto Oncogene Proteins,Proto-Oncogene Proteins, Cellular,c onc Proteins
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D016159 Tumor Suppressor Protein p53 Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER. p53 Tumor Suppressor Protein,Cellular Tumor Antigen p53,Oncoprotein p53,TP53 Protein,TRP53 Protein,p53 Antigen,pp53 Phosphoprotein,Phosphoprotein, pp53

Related Publications

Hiroaki Suzuki, and Megumi Kurita, and Kiyohisa Mizumoto, and Ikuo Nishimoto, and Etsuro Ogata, and Masaaki Matsuoka
BAX enhances paclitaxel-induced apoptosis through a p53-independent pathway.
November 1996, Proceedings of the National Academy of Sciences of the United States of America,
Hiroaki Suzuki, and Megumi Kurita, and Kiyohisa Mizumoto, and Ikuo Nishimoto, and Etsuro Ogata, and Masaaki Matsuoka
p53-independent apoptosis is induced by the p19ARF tumor suppressor.
July 2002, Biochemical and biophysical research communications,
Hiroaki Suzuki, and Megumi Kurita, and Kiyohisa Mizumoto, and Ikuo Nishimoto, and Etsuro Ogata, and Masaaki Matsuoka
Histone deacetylase inhibitors prevent p53-dependent and p53-independent Bax-mediated neuronal apoptosis through two distinct mechanisms.
March 2009, The Journal of neuroscience : the official journal of the Society for Neuroscience,
Hiroaki Suzuki, and Megumi Kurita, and Kiyohisa Mizumoto, and Ikuo Nishimoto, and Etsuro Ogata, and Masaaki Matsuoka
HGF-mediated apoptosis via p53/bax-independent pathway activating JNK1.
April 1999, Carcinogenesis,
Hiroaki Suzuki, and Megumi Kurita, and Kiyohisa Mizumoto, and Ikuo Nishimoto, and Etsuro Ogata, and Masaaki Matsuoka
Multiple domains of the mouse p19ARF tumor suppressor are involved in p53-independent apoptosis.
February 2003, Biochemical and biophysical research communications,
Hiroaki Suzuki, and Megumi Kurita, and Kiyohisa Mizumoto, and Ikuo Nishimoto, and Etsuro Ogata, and Masaaki Matsuoka
p53-independent apoptosis induced by paclitaxel through an indirect mechanism.
September 1997, Proceedings of the National Academy of Sciences of the United States of America,
Hiroaki Suzuki, and Megumi Kurita, and Kiyohisa Mizumoto, and Ikuo Nishimoto, and Etsuro Ogata, and Masaaki Matsuoka
Ganciclovir-induced ablation non-proliferating thyrocytes expressing herpesvirus thymidine kinase occurs by p53-independent apoptosis.
July 1996, Oncogene,
Hiroaki Suzuki, and Megumi Kurita, and Kiyohisa Mizumoto, and Ikuo Nishimoto, and Etsuro Ogata, and Masaaki Matsuoka
Cytoplasmic p53 and activated Bax regulate p53-dependent, transcription-independent neural precursor cell apoptosis.
March 2010, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
Hiroaki Suzuki, and Megumi Kurita, and Kiyohisa Mizumoto, and Ikuo Nishimoto, and Etsuro Ogata, and Masaaki Matsuoka
Hsp90 inhibitors promote p53-dependent apoptosis through PUMA and Bax.
November 2013, Molecular cancer therapeutics,
Hiroaki Suzuki, and Megumi Kurita, and Kiyohisa Mizumoto, and Ikuo Nishimoto, and Etsuro Ogata, and Masaaki Matsuoka
Adenovirus-mediated overexpression of p14(ARF) induces p53 and Bax-independent apoptosis.
May 2002, Oncogene,
Copied contents to your clipboard!