Zonal expression of the thyroid hormone receptor alpha isoforms in rodent liver. 2003

B Zandieh-Doulabi, and E Dop, and M Schneiders, and M P-T Schiphorst, and A Mansen, and B Vennström, and C D Dijkstra, and O Bakker, and W M Wiersinga
Department of Endocrinology and Metabolism, Academic Medical Centre, F5-171, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.

Many metabolic processes occur simultaneously in the liver in different locations along the porto-central axis of the liver units. These processes are often regulated by hormones, one of which is thyroid hormone which for its action depends on the presence of the different isoforms of the thyroid hormone receptor (TR). These are encoded by two genes: c-erbA-alpha encoding TRalpha1 and TRalpha2 and their respective Delta isoforms, and c-erbA-beta which encodes TRbeta1, TRbeta2 and TRbeta3. We recently found a zonal (pericentral) expression of and a diurnal variation in the TRbeta1 isoform in rat liver. We were therefore also interested to see whether TRalpha1 and TRalpha2 expression showed similar characteristics. For this reason we raised both polyclonal and monoclonal antibodies against TRalpha1 and TRalpha2 isoforms and characterised these. Antibody specificity was tested using Western blots and immunohistochemistry in liver of TR isoform-specific knockout animals. Using these antibodies we found that the TRalpha1 and TRalpha2 isoforms are zonally expressed around the central vein in rat liver. The experiments show that the portal to central gradient of TRalpha1 is broader than that of TRbeta1. Moreover, the expression of the TRalpha2 protein showed a diurnal variation with a peak in the afternoon when the animals are least active whereas no such variation was found for the TRalpha1 protein. From our data it appears that both the TRalpha1 and TRalpha2 isoforms show a zonal distribution in liver. This finding, together with the observed diurnal rhythm, has major implications for interpreting and timing experiments concerning the TR and its downstream actions in liver.

UI MeSH Term Description Entries
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D002940 Circadian Rhythm The regular recurrence, in cycles of about 24 hours, of biological processes or activities, such as sensitivity to drugs or environmental and physiological stimuli. Diurnal Rhythm,Nyctohemeral Rhythm,Twenty-Four Hour Rhythm,Nycthemeral Rhythm,Circadian Rhythms,Diurnal Rhythms,Nycthemeral Rhythms,Nyctohemeral Rhythms,Rhythm, Circadian,Rhythm, Diurnal,Rhythm, Nycthemeral,Rhythm, Nyctohemeral,Rhythm, Twenty-Four Hour,Rhythms, Circadian,Rhythms, Diurnal,Rhythms, Nycthemeral,Rhythms, Nyctohemeral,Rhythms, Twenty-Four Hour,Twenty Four Hour Rhythm,Twenty-Four Hour Rhythms
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000918 Antibody Specificity The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site. Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D020033 Protein Isoforms Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING. Isoform,Isoforms,Protein Isoform,Protein Splice Variant,Splice Variants, Protein,Protein Splice Variants,Isoform, Protein,Isoforms, Protein,Splice Variant, Protein,Variant, Protein Splice,Variants, Protein Splice
D037021 Thyroid Hormone Receptors alpha High affinity receptors for THYROID HORMONES, especially TRIIODOTHYRONINE. These receptors are usually found in the nucleus where they regulate DNA transcription. They are encoded by the THRA gene (also known as NR1A1, THRA1, ERBA or ERBA1 gene) as several isoforms produced by alternative splicing. Proto-Oncogene Proteins c-erbA,Receptors alpha, Thyroid Hormone,c-erbA Protein,erbA Proto-Oncogene Products,ERBA1 Gene Products,NR1A1 Gene Products,THRA Gene Products,Thyroid Hormone Receptor alpha-1,Thyroid Hormone Receptor alpha-2,c-erb A Protein,c-erb A Proteins,c-erbA Proteins,c-erbA alpha,c-erbA alpha Protein,c-erbA-1 Protein,Gene Products, ERBA1,Gene Products, NR1A1,Gene Products, THRA,Proto Oncogene Proteins c erbA,Proto-Oncogene Products, erbA,Thyroid Hormone Receptor alpha 1,Thyroid Hormone Receptor alpha 2,c erb A Protein,c erb A Proteins,c erbA 1 Protein,c erbA Protein,c erbA Proteins,c erbA alpha,c erbA alpha Protein,c-erbA, Proto-Oncogene Proteins,erbA Proto Oncogene Products

Related Publications

B Zandieh-Doulabi, and E Dop, and M Schneiders, and M P-T Schiphorst, and A Mansen, and B Vennström, and C D Dijkstra, and O Bakker, and W M Wiersinga
September 1996, Biochemical and biophysical research communications,
B Zandieh-Doulabi, and E Dop, and M Schneiders, and M P-T Schiphorst, and A Mansen, and B Vennström, and C D Dijkstra, and O Bakker, and W M Wiersinga
May 2003, Molecular and cellular endocrinology,
B Zandieh-Doulabi, and E Dop, and M Schneiders, and M P-T Schiphorst, and A Mansen, and B Vennström, and C D Dijkstra, and O Bakker, and W M Wiersinga
April 2004, Molecular and cellular endocrinology,
B Zandieh-Doulabi, and E Dop, and M Schneiders, and M P-T Schiphorst, and A Mansen, and B Vennström, and C D Dijkstra, and O Bakker, and W M Wiersinga
May 2004, Neurochemical research,
B Zandieh-Doulabi, and E Dop, and M Schneiders, and M P-T Schiphorst, and A Mansen, and B Vennström, and C D Dijkstra, and O Bakker, and W M Wiersinga
December 2008, Surgical neurology,
B Zandieh-Doulabi, and E Dop, and M Schneiders, and M P-T Schiphorst, and A Mansen, and B Vennström, and C D Dijkstra, and O Bakker, and W M Wiersinga
August 1991, Molecular endocrinology (Baltimore, Md.),
B Zandieh-Doulabi, and E Dop, and M Schneiders, and M P-T Schiphorst, and A Mansen, and B Vennström, and C D Dijkstra, and O Bakker, and W M Wiersinga
July 2005, American journal of physiology. Endocrinology and metabolism,
B Zandieh-Doulabi, and E Dop, and M Schneiders, and M P-T Schiphorst, and A Mansen, and B Vennström, and C D Dijkstra, and O Bakker, and W M Wiersinga
April 2006, Endocrine journal,
B Zandieh-Doulabi, and E Dop, and M Schneiders, and M P-T Schiphorst, and A Mansen, and B Vennström, and C D Dijkstra, and O Bakker, and W M Wiersinga
May 1993, The Journal of biological chemistry,
B Zandieh-Doulabi, and E Dop, and M Schneiders, and M P-T Schiphorst, and A Mansen, and B Vennström, and C D Dijkstra, and O Bakker, and W M Wiersinga
October 2007, Endocrinology,
Copied contents to your clipboard!