BIOMAT Database Logo BIOMAT Database Logo

Preparation of latex sensitized with rabbit IgG antibody for slide reversed passive agglutination. 1992

S Yamamoto, and K Tagata, and Y Ishikawa, and H Fujise, and H Nagahata, and M Yamada, and T Sakano, and M Morimatsu, and M Naiki
Laboratory of Immunology, Faculty of Environmental Health, Azabu University, Kanagawa, Japan.

A method is described for preparing latex particles sensitized with IgG antibody (IgG-sensitized latex) applicable to the slide reversed passive agglutination (RPLA) test. Soap-free latex (latex) was sensitized with IgG which had been isolated from rabbit anti-bovine lactoferrin serum using protein A Sepharose CL 4B. Unadsorbed protein-binding sites on the surface of latex were blocked with bovine serum albumin (BSA). IgG-sensitized latex that gave better agglutination in RPLA could be selectively obtained by centrifugation at 19 900g for 15 min in 0.01 mol/L glycine buffer (pH 7.3; specific gravity 1.042) containing 3% NaCl, 5% saccharose and 2% choline chloride. By dispersing this IgG-sensitized latex in 0.01 mol/L glycine buffer (pH 7.3) containing 1-2% BSA, a uniformly suspended, highly reactive, readily agglutinable preparation was obtained.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007841 Latex Fixation Tests Passive agglutination tests in which antigen is adsorbed onto latex particles which then clump in the presence of antibody specific for the adsorbed antigen. (From Stedman, 26th ed) Latex Agglutination Tests,Agglutination Test, Latex,Agglutination Tests, Latex,Fixation Test, Latex,Fixation Tests, Latex,Latex Agglutination Test,Latex Fixation Test,Test, Latex Agglutination,Test, Latex Fixation,Tests, Latex Agglutination,Tests, Latex Fixation
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D000327 Adsorption The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily. Adsorptions
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

S Yamamoto, and K Tagata, and Y Ishikawa, and H Fujise, and H Nagahata, and M Yamada, and T Sakano, and M Morimatsu, and M Naiki
Reversed passive latex agglutination assay for detection of toxigenic Corynebacterium diphtheriae.
December 1997, Journal of clinical microbiology,
S Yamamoto, and K Tagata, and Y Ishikawa, and H Fujise, and H Nagahata, and M Yamada, and T Sakano, and M Morimatsu, and M Naiki
A latex slide agglutination test for rapid detection of antimyeloperoxidase antibody.
October 1999, Journal of clinical pathology,
S Yamamoto, and K Tagata, and Y Ishikawa, and H Fujise, and H Nagahata, and M Yamada, and T Sakano, and M Morimatsu, and M Naiki
An assessment of a latex agglutination slide test for toxoplasma antibody.
April 1972, Journal of clinical pathology,
S Yamamoto, and K Tagata, and Y Ishikawa, and H Fujise, and H Nagahata, and M Yamada, and T Sakano, and M Morimatsu, and M Naiki
Determination of Clostridium botulinum toxin by reversed passive latex agglutination.
August 1984, Nihon juigaku zasshi. The Japanese journal of veterinary science,
S Yamamoto, and K Tagata, and Y Ishikawa, and H Fujise, and H Nagahata, and M Yamada, and T Sakano, and M Morimatsu, and M Naiki
Detection of Clostridium difficile toxin A by reversed passive latex agglutination.
January 1999, Microbiology and immunology,
S Yamamoto, and K Tagata, and Y Ishikawa, and H Fujise, and H Nagahata, and M Yamada, and T Sakano, and M Morimatsu, and M Naiki
Latex slide agglutination test for Aspergillus antibodies.
November 1970, Sabouraudia,
S Yamamoto, and K Tagata, and Y Ishikawa, and H Fujise, and H Nagahata, and M Yamada, and T Sakano, and M Morimatsu, and M Naiki
Evaluation of a Reversed Passive Latex Agglutination Test Kit for Clostridium perfringens Enterotoxin.
July 1986, Journal of food protection,
S Yamamoto, and K Tagata, and Y Ishikawa, and H Fujise, and H Nagahata, and M Yamada, and T Sakano, and M Morimatsu, and M Naiki
Development of reversed passive latex agglutination for detection of Thai cobra (Naja kaouthia) venom.
June 1999, Journal of natural toxins,
S Yamamoto, and K Tagata, and Y Ishikawa, and H Fujise, and H Nagahata, and M Yamada, and T Sakano, and M Morimatsu, and M Naiki
[Detection of heat-labile enterotoxin produced by E. coli with reversed passive latex agglutination].
May 1989, Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology,
S Yamamoto, and K Tagata, and Y Ishikawa, and H Fujise, and H Nagahata, and M Yamada, and T Sakano, and M Morimatsu, and M Naiki
Detection of toxoplasma IgM antibody by passive latex agglutination reaction.
August 1987, Journal of immunological methods,
Copied contents to your clipboard!