A sampling method for comparing fungal concentrations in carpets. 2003

Joe C Spurgeon
Bayshore Environmental, Inc., 1349 Montevideo Ave., Placentia, CA 92870; jospur@mindspring.com

A microvacuum method is described for sampling fungal contaminants in carpet dust and reporting the results on an area basis. When sampling parameters such as suction force, contact time, and area sampled were held constant, and the results were reported on an area basis, fungal concentrations were associated with the potential for water intrusion, a determinant of exposure. Carpet dust samples were collected in open-face 25-mm cassettes containing 0.8 micro m mixed cellulose ester filters. The airflow rate was calibrated at 10 L/min, and the open-faced cassette was held firmly against the carpet at 20 separate spots for a period of 5 sec at each spot. An area of 98 cm(2) of carpet was sampled with each cassette. A total of 58 carpet dust samples were collected in 31 residential condominium units using the described methodology. The carpets were stratified into three groups: (1) controls and those at centers of rooms, (2) at sliding glass doors and under windows, and (3) in areas of water intrusion reported by the occupant. The geometric mean concentrations (GM) of total fungi increased in the listed order, as did the GM concentrations of Penicillium spp. and Aspergillus spp. detected in the samples. In addition, the 95% confidence intervals on the GM concentrations for total fungi could be used to classify the carpets into three groups: uncontaminated, potentially contaminated, and contaminated.

UI MeSH Term Description Entries
D002138 Calibration Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output. Calibrations
D004391 Dust Earth or other matter in fine, dry particles. (Random House Unabridged Dictionary, 2d ed) House Dust,Housedust,Dust, House
D004784 Environmental Monitoring The monitoring of the level of toxins, chemical pollutants, microbial contaminants, or other harmful substances in the environment (soil, air, and water), workplace, or in the bodies of people and animals present in that environment. Monitoring, Environmental,Environmental Surveillance,Surveillance, Environmental
D005430 Floors and Floorcoverings The surface of a structure upon which one stands or walks. Carpets,Floorcoverings,Floors,Carpet,Floor,Floorcovering,Floorcoverings and Floors
D005658 Fungi A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies. Fungi, Filamentous,Molds,Filamentous Fungi,Filamentous Fungus,Fungus,Fungus, Filamentous,Mold
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013048 Specimen Handling Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation. Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings
D014618 Vacuum A space in which the pressure is far below atmospheric pressure so that the remaining gases do not affect processes being carried on in the space. Vacuums
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D016902 Air Pollution, Indoor The contamination of indoor air. Air Quality, Indoor,Indoor Air Pollution,Indoor Air Quality,Pollution, Indoor Air

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